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INVESTIGATION OF miRNAS POTENTIALLY INVOLED IN THE SLC2A4-GLUT4 EXPRESSION REGULATION IN SKELETAL MUSCLE OF DIABETIC RATS

Grant number: 12/20432-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2013
Effective date (End): August 31, 2016
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Ubiratan Fabres Machado
Grantee:João Victor Del Conti Esteves
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:12/04831-1 - New players in glycemic control and chronic complications of Diabetes mellitus: preventive and therapeutic perspectives, AP.TEM

Abstract

Diabetes Mellitus (DM) is a metabolic disorder of high prevalence in the world, responsible for numerous co-morbidities, generating costly expenditures on health systems. Recently, pivotal role was attributed to the benefits of physical exercise for the prevention and/or treatment of DM, especially involving the improvement of glucose uptake in skeletal muscle, however, the molecular mechanisms involved in this process are still obscure. Recently, a new element appears in the pathophysiology of DM, which has received high importance: it is a group of small RNAs that do not encode proteins, but contribute to alterations in the expression of genes, called microRNAs (miRNAs). MiRNAs generally inhibit protein translation, and can affect the stability of mRNAs. Its effects have been observed in several cellular dysfunctions related to insulin secretion and glucose homeostasis, and consequently the development of DM. As far we know, no study has evaluated the influence of exercise on the expression of these miRNAs in muscle tissue of diabetic or non-diabetics animals. Therefore, the objectives of this study are: to investigate the expression of miR-1, miR-133a, miR-133b and miR-206 in skeletal muscle of diabetic rats and controls, as well as studying the effect of muscle contraction in vitro on expression of these miRNAs, and investigate the possible correlation between the variations of miRNAs and the expression of glucose transporter GLUT4 in skeletal muscle. It is expected that the results will help to understand the effects of muscle contraction on GLUT4 expression, generating new prospects for inducing increased GLUT4, improving the capacity of glucose utilization in muscle from diabetes bearers.

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
JULIO, URSULA F.; PANISSA, VALERIA L. G.; CURY, RUBIANA L.; AGOSTINHO, MARCUS F.; ESTEVES, JOAO V. D. C.; FRANCHINI, EMERSON. Energy System Contributions in Upper and Lower Body Wingate Tests in Highly Trained Athletes. RESEARCH QUARTERLY FOR EXERCISE AND SPORT, v. 90, n. 2 MAR 2019. Web of Science Citations: 0.
YONAMINE, CAIO Y.; ALVES-WAGNER, ANA B.; ESTEVES, V, JOAO; OKAMOTO, MARISTELA M.; CORREA-GIANNELLA, MARIA L.; GIANNELLA-NETO, DANIEL; MACHADO, UBIRATAN F. Diabetes induces tri-methylation at lysine 9 of histone 3 at Slc2a4 gene in skeletal muscle: A new target to improve glycemic control. Molecular and Cellular Endocrinology, v. 481, p. 26-34, FEB 5 2019. Web of Science Citations: 0.
ESTEVES, V, JOAO; YONAMINE, CAIO Y.; PINTO-JUNIOR, DANILO C.; GERLINGER-ROMERO, FREDERICO; ENGUITA, FRANCISCO J.; MACHADO, UBIRATAN F. Diabetes Modulates MicroRNAs 29b-3p, 29c-3p, 199a-5p and 532-3p Expression in Muscle: Possible Role in GLUT4 and HK2 Repression. FRONTIERS IN ENDOCRINOLOGY, v. 9, SEP 12 2018. Web of Science Citations: 6.
PINTO-JUNIOR, DANILO C.; SILVA, KAROLLINE S.; MICHALANI, MARIA L.; YONAMINE, CAIO Y.; ESTEVES, JOAO V.; FABRE, NELLY T.; THIEME, KARINA; CATANOZI, SERGIO; OKAMOTO, MARISTELA M.; SERAPHIM, PATRICIA M.; CORREA-GIANNELLA, MARIA L.; PASSARELLI, MARISA; MACHADO, UBIRATAN F. Advanced glycation end products-induced insulin resistance involves repression of skeletal muscle GLUT4 expression. SCIENTIFIC REPORTS, v. 8, MAY 25 2018. Web of Science Citations: 4.
ESTEVES, JOAO VICTOR; ENGUITA, FRANCISCO JAVIER; MACHADO, UBIRATAN FABRES. MicroRNAs-Mediated Regulation of Skeletal Muscle GLUT4 Expression and Translocation in Insulin Resistance. JOURNAL OF DIABETES RESEARCH, 2017. Web of Science Citations: 16.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ESTEVES, João Victor Del Conti. microRNAs 29b, 29c, 199a e 532-3p are potentials repressors of GLUT4 and HK2 expression in skeletal muscle of diabetic rats.. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas São Paulo.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.