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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga

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Author(s):
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Tonon, Luciane A. Chimetto [1, 2] ; Rua, Cintia [1, 2] ; Crnkovic, Camila M. [1, 3] ; Bernardi, I, Darlon ; Junior, Osmindo R. Pires [4] ; Haddad, Celio F. B. [5, 6] ; Pedrosa, Carolinada S. G. [7] ; Souza, Leticia R. Q. [7] ; Rehen, Stevens K. [7, 8] ; de Azevedo, Gustavo P. R. [2] ; Thompson, Cristiane C. [2] ; Thompson, Fabiano L. [2] ; Berlinck, Roberto G. S. [9]
Total Authors: 13
Affiliation:
[1] Univ Sao Paulo, Inst Quim Sao Carlos, CP 780, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Rio de Janeiro UFRJ, Inst Biol, SAGE COPPE, Rio De Janeiro, RJ - Brazil
[3] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Tecnol Bioquim Farmaceut FBT, Sao Paulo, SP - Brazil
[4] Univ Brasilia, Inst Biol, Lab Toxinol, Brasilia, DF - Brazil
[5] Univ Estadual Paulista, Dept Biodiversidade, Inst Biociencias, UNESP, Rio Claro, SP - Brazil
[6] Univ Estadual Paulista, Ctr Aquicultura, Inst Biociencias, UNESP, Rio Claro, SP - Brazil
[7] Inst Or Pesquisa & Ensino IDOR, Rio De Janeiro, RJ - Brazil
[8] Univ Fed Rio de Janeiro UFRJ, Inst Ciencias Biomed, Rio De Janeiro, RJ - Brazil
[9] Bernardi, Darlon, I, Univ Sao Paulo, Inst Quim Sao Carlos, CP 780, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 9
Document type: Journal article
Source: Toxicon; v. 203, p. 139-146, NOV 2021.
Web of Science Citations: 1
Abstract

The genus Brachycephalus includes small species of aposematic anurans known as microendemic, occurring in the mountains of the Atlantic Forest. Brachycephalus ephippium, B. nodoterga and B. pernix have been reported to contain the neurotoxin tetrodotoxin in skin and viscera. The biological conservation of several Brachycephalus species is currently threatened by climate change, deforestation, and the pandemic caused by the fungus Batrachochytrium dendrobatidis (Bd). Despite the well-known importance of amphibians' associated bacteria in the defensive role against pathogens, there is still a poor understanding of amphibian microbiome composition. The present study investigated the composition of B. pitanga microbial community and the presence of TTX in the host and in cultures of bacterial isolates, using a combination of metagenomics, bacterial culture isolation, mass spectrometry and metabolomic analyses. Results of culture-dependent and-independent analyses characterized the microbial communities associated with the skin and viscera of B. pitanga. Mass spectrometry analysis indi-cated the presence of TTX in host tissues, while bacterial production of TTX was not observed under the experimental conditions used in this investigation. This is the first report confirming the occurrence of TTX in B. pitanga. (AU)

FAPESP's process: 13/50741-7 - Diversity and conservation of Brazilian amphibians
Grantee:Célio Fernando Baptista Haddad
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 16/14375-4 - Omics tools applied to the discovery of tetrodotoxin producing organisms and biosynthetic genes
Grantee:Luciane Alessandra Chimetto Tonon
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/05133-7 - Study of marine-derived microrganisms metabolism: chemical profile and biosynthesis
Grantee:Laura Pavan Ióca
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/06014-4 - New secondary metabolites of endophytic and marine organisms as proteasome inhibitors: a validated target against cancer
Grantee:Juliana Regina Gubiani
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/50228-8 - Biodiversity components, and their metabolic characters, of Brazilian Islands: an integrated approach
Grantee:Roberto Gomes de Souza Berlinck
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 16/21341-9 - Hydrophilic and bioactive secondary metabolites produced by filamentous fungi
Grantee:Darlon Irineu Bernardi
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 19/00447-1 - Improving and diversifying the production and investigating the biosynthesis of phomactins by the cultured fungus Biatriospora sp. CBMAI 1333
Grantee:Camila Manoel Crnkovic
Support Opportunities: Scholarships in Brazil - Post-Doctoral