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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bacterial diversity in organic and conventional Minas Frescal cheese production using targeted 16S rRNA sequencing

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da Silva Abreu, Anderson Clayton [1] ; Carazzolle, Marcelo Falsarella [2] ; Crippa, Bruna Lourenco [1] ; Barboza, Giovana Rueda [1] ; Rall, Vera Lucia Mores [3] ; Rocha, Liliana de Oliveira [1] ; Cirone Silva, Nathalia Cristina [1]
Total Authors: 7
[1] Univ Estadual Campinas, Sch Food Engn FEA, Dept Food Sci, BR-13083862 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol IB, Dept Genet & Mol Biol, BR-13083862 Campinas, SP - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biol Sci, BR-18618691 Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 0

Molecular identification and the study of bacteria in processed food products can be challenging or suffer limitations when using conventional methods. However, with Next Generation Sequencing (NGS), one can identify bacterial species with a significantly higher detection sensitivity using a 16S rRNA target gene. This study aimed to characterise the bacterial diversity of the organic and conventional production of Minas Frescal cheese, through the NGS analysis of the 16S rRNA gene. DNA was extracted from 96 samples, 48 from organic production and 48 from conventional production. The bacterial families and genera with the highest prevalence were identified. The most prevalent families were Enterobacteriaceae, Planococcaceae and Moraxellaceae. The NGS 16S rRNA tool proved to be very useful for bacterial characterisation, but it is still not possible to say whether a product contains more or less bacteria based on organic production, as there are several factors that contribute to contamination. (c) 2021 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 16/02118-7 - Molecular biology tools to study microbial diversity and characterization of Staphylococcus aureus in organic and conventional dairy in São Paulo
Grantee:Nathalia Cristina Cirone Silva
Support type: Regular Research Grants
FAPESP's process: 18/00986-7 - Coagulase-negative Staphylococcus: analyze of the resistance profile and enterotoxigenic in strains isolated from dairy products
Grantee:Luiz Gustavo de Matos
Support type: Scholarships in Brazil - Scientific Initiation