| Full text | |
| Author(s): |
Michelin, E. C.
[1]
;
Bedoya-Serna, C. M.
[1]
;
Carrion, L. C. S.
[1]
;
Levy-Pereira, N.
[1]
;
Cury, F. S.
[1]
;
Passarelli, D.
[1]
;
Lima, C. G.
[2]
;
Yasui, G. S.
[3]
;
Sousa, R. L. M.
[1]
;
Fernandes, A. M.
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo FZEA USP, Fac Zootecnia & Engn Alimentos, Dept Med Vet, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Sao Paulo FZEA USP, Fac Zootecnia & Engn Alimentos, Dept Ciencias Basicas, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[3] Inst Chico Mendes Conservacao Biodiversidade ICMB, Rodovia SP-201, Km 6-5, BR-13630970 Pirassununga, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | WORLD MYCOTOXIN JOURNAL; v. 14, n. 3, SI, p. 421-430, 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
The objective of this study was to evaluate biochemical parameters and histopathology of liver in Matrinxa (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish chronically exposed to dietary aflatoxins. Fish feed was artificially contaminated with aflatoxins and the treatments were: Control - feed without toxin; Treatment A - feed + 10 mu g aflatoxin B-1 (AFB(1))/kg; Treatment B - feed + 20 mu g AFB(1)/kg; and Treatment C - feed + 50 mu g AFB(1)/kg. Matrinxa and Pacu juvenile fish were placed in tanks for 180 days. Five experimental units per treatment were monthly sampled and submitted to blood collection and removal of hepatic tissue. Thus, twenty blood and liver samples for each species were collected monthly, adding up to 240 samples analysed. To verify biochemical changes, analyses included total proteins, albumin, globulins, aspartate aminotransferase (AST) and alkaline phosphatase (ALP). The hepatic tissue was examined microscopically and the slides presenting histopathological changes were photo-documented. There was effect of treatment (P<0.05) for AST and ALP in Matrinxa, while no effect (P>0.05) was observed in Pacu. A reduction (P<0.05) in AST and ALP values during the time of exposure was observed in all treatments for both species. Fatty degeneration and liver damage were observed for both species in treatments exposed to aflatoxins. Fatty degeneration in Pacu was noticed after 30 days of exposure, while in Matrinxa it was observed after 60 days. Disorganisation of the hepatocyte cord arrangement was also observed in those treatments exposed to aflatoxin, following 90 days of exposure in Matrinxa, and after 60 days in Pacu. Therefore, aflatoxins have little influence on biochemical parameters in the species evaluated. However, exposure to aflatoxins caused liver changes, such as cell death, fatty and hydropic degeneration, thus it could be concluded that both species are susceptible to the toxic effects of long-term exposure to dietary AFB(1). (AU) | |
| FAPESP's process: | 13/23556-4 - Evaluation of aflatoxins transfer from feed to commercial species of fish and occurrence of aflatoxins in fish farming from State of São Paulo |
| Grantee: | Andrezza Maria Fernandes |
| Support Opportunities: | Regular Research Grants |