Induction of resistance mechanisms in Rhodotorula toruloides for growth in sugarcane hydrolysate with high inhibitor content

Author(s)

Santos Lopes, Helberth Junnior ^[1] ; Bonturi, Nemailla ^[2] ; Miranda, Everson Alves ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Chem Engn, Dept Mat & Bioproc Engn, BR-13083852 Campinas, SP - Brazil

^[2] Univ Tartu, Inst Technol, Tartu - Estonia

• Document type: Journal article
• Source: Applied Microbiology and Biotechnology; v. 105, n. 24 NOV 2021.
• Web of Science Citations: 0

Abstract

The oleaginous yeast Rhodotorula toruloides is a potential lipid producer for biodiesel production. However, this yeast shows growth inhibition due to harmful compounds when cultivated in hemicellulose hydrolysate. Here, we present a comparative analysis of colony selection and heterologous adaptive laboratory enhancement (ALE) strategies for obtaining robust strains. We implemented these ALE strategies for R. toruloides in a culture medium containing sugarcane hemicellulose hydrolysate. Our comparison study showed that the strain obtained with heterogeneous ALE strategy (Rth) reached a mu(max) of 55% higher than the parental strain. It also exhibited higher biomass production (6.51 g/l) and lipid content (60%). ALE with colony selection strategy (Rtc) had a fitness gain in terms of shortening of the lag phase (9 h) when compared to Rth and parental strain (11.67, 12.33 h, respectively). When cultivated in Eucalyptus urograndis hemicellulose hydrolysate, the Rth strain achieved a high lipid content, 64%. Kinetics studies showed a strong effect of acetic acid as a repressor of xylose consumption during R. toruloides cultivation. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants