| Full text | |
| Author(s): |
da Mota Araujo, Helena Rachel
[1]
;
Sartori, Marina Rincon
[2]
;
Navarro, Claudia D. C.
[2]
;
de Carvalho, Jose Eduardo
[3]
;
da Cruz, Andre Luis
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Fed Bahia, Inst Biol, Campus Ondina, BR-40170115 Salvador, BA - Brazil
[2] Univ Estadual Campinas, Fac Ciencias Med, Dept Patol, BR-13083877 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Inst Ciencias Quim Ambientais & Farmaceut, Campus Diadema, BR-04021001 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Journal of Experimental Biology; v. 224, n. 21 NOV 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Snakes are interesting examples of taxa that can overcome energy metabolism challenges, as many species can endure long periods without feeding, and their eventual meals are of reasonably large sizes, thus exhibiting dual extreme adaptations. Consequently, metabolic rate increases considerably to attend to the energetic demand of digestion, absorption and protein synthesis. These animals should be adapted to transition from these two opposite states of energy fairly quickly, and therefore we investigated mitochondrial function plasticity in these states. Herein, we compared liver mitochondrial bioenergetics of the boid snake Boa constrictor during fasting and after meal intake. We fasted the snakes for 60 days, and then we fed a subgroup with 30% of their body size and evaluated their maximum postprandial response. We measured liver respiration rates from permeabilized tissue and isolated mitochondria. From isolated mitochondria, we also measured Ca2+ retention capacity and redox status. Mitochondrial respiration rates were maximized after feeding, reaching an approximately 60% increase from fasting levels when energized with complex I-linked substrates. Interestingly, fasting and fed snakes exhibited similar respiratory control ratios and citrate synthase activity. Furthermore, we found no differences in Ca2+ retention capacity, indicating no increase in susceptibility to mitochondrial permeability transition, and no changes in mitochondrial redox state, although fed animals exhibited increases in the release of H2O2. Thus, we conclude that liver mitochondria from B. constrictor snakes increase respiration rates during the postprandial period and quickly improve the bioenergetic capacity without compromising redox balance. (AU) | |
| FAPESP's process: | 08/57712-4 - The National Institute of Comparative Physiological Research |
| Grantee: | Augusto Shinya Abe |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 20/12962-5 - A look on the habitat heterogeneity: how did the physiology and behavior of tropidurine lizards allow occupation of forests and open environments? |
| Grantee: | José Eduardo de Carvalho |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/05487-6 - Mitochondrial function and molecular markers associated with aging in the long-lived red-footed tortoise Chelonoidis carbonaria |
| Grantee: | Marina Rincon Sartori |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |