| Full text | |
| Author(s): |
Neto, Mario de Oliveira
[1]
;
Fernandes, Adriano de Freitas
[2]
;
Piiadov, Vassili
[2]
;
Craievich, Aldo Felix
[3]
;
de Araujo, Evandro Ares
[4]
;
Polikarpov, Igor
[2]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Paulista, Biosci Inst, Biophys & Pharmacol Dept, Botucatu, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Inst Phys, Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil
[3] Univ Sao Paulo, Dept Appl Phys, Sao Paulo, SP - Brazil
[4] Brazilian Ctr Res Energy & Mat, Brazilian Synchrotron Light Lab, Campinas, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Protein Science; v. 31, n. 1, SI NOV 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
SAXSMoW (SAXS Molecular Weight) is an online platform widely used over the past few years for determination of molecular weights of proteins in dilute solutions. The scattering intensity retrieved from small-angle X-ray scattering (SAXS) raw data is the sole input to SAXSMoW for determination of molecular weights of proteins in liquid. The current updated SAXSMoW version 3.0 determines the linear dependence of the true protein volume on their apparent protein volume, based on SAXS curves calculated for 67,000 protein structures selected from the Protein Data Bank. SAXSMoW 3.0 was tested against 43 experimental SAXS scattering curves from proteins with known molecular weights. Our results demonstrate that most of the molecular weights determined for the nonglycosylated and also for the glycosylated proteins are in good agreement with their expected molecular weights. Additionally, the average discrepancies between the calculated molecular weights and their nominal values for glycosylated proteins are similar to those for nonglycosylated ones. (AU) | |
| FAPESP's process: | 18/22300-0 - Synergistic activities to boost enzymatic lignocellulose oxidation using light and LPMOs |
| Grantee: | Igor Polikarpov |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation |
| Grantee: | Igor Polikarpov |
| Support Opportunities: | Research Projects - Thematic Grants |