Design for preparation of more active cross-linked enzyme aggregates ofBurkholderia cepacialipase using palm fiber residue

Author(s)

Alves, Nanda R. ^[1] ; Pereira, Matheus M. ^[2] ; Giordano, Raquel L. C. ^[3] ; Tardioli, Paulo W. ^[3] ; Lima, Alvaro S. ^{[1, 4]} ; Soares, Cleide M. F. ^{[1, 4]} ; Souza, Ranyere L. ^{[1, 4]}

Affiliation

^[1] Univ Tiradentes, UNIT, Av Murilo Dantas 300, Aracaju, SE - Brazil

^[2] Univ Aveiro, Chem Dept, CICECO, Campus Univ Santiago Aveiro, P-3810193 Aveiro - Portugal

^[3] Univ Fed Sao Carlos DEQ UFSCar, Dept Engn Quim, Programa Posgrad Engn Quim PPG EQ, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[4] Inst Tecnol & Pesquisa, ITP, Av Murilo Dantas 300, Aracaju, SE - Brazil

• Document type: Journal article
• Source: Bioprocess and Biosystems Engineering; v. 44, n. 1, p. 57-66, JAN 2021.
• Web of Science Citations: 6

Abstract

A new design of cross-linked enzyme aggregates (CLEAs) ofBurkholderia cepacialipase (BCL) based mainly on the use of lignocellulosic residue of palm fiber as an additive was proposed. Different parameters for the preparation of active CLEAs in the hydrolysis of olive oil, such as precipitation agents, crosslinking agent concentration, additives, and coating agents were investigated. The highest activity yield (121.1 +/- 0.1%) and volumetric activity (1578.1 +/- 2.5 U/mL) were achieved for CLEAs prepared using the combination of a coating step with Triton(R)X-100 and polyethyleneimine plus the use of palm fiber as an additive. The variations of the secondary structures of BCL-CLEAs were analyzed by second-derivative infrared spectra, mainly indicating a reduction of the alpha-helix structure, which was responsible for the lipase activation in the supramolecular structure of the CLEAs. Thus, these results provided evidence of an innovative design of BCL-CLEAs as a sustainable and biocompatible opportunity for biotechnology applications. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants