Highly sensitive and selective acute myocardial infarction detection using aptamer-tethered MoS2 nanoflower and screen-printed electrodes

Author(s)

Vasudevan, Mugashini ^{[1, 2]} ; Tai, Melvin J. Y. ^{[1, 2]} ; Perumal, Veeradasan ^{[1, 2]} ; Gopinath, Subash C. B. ^{[3, 4]} ; Murthe, Satisvar Sundera ^{[1, 5]} ; Ovinis, Mark ^[2] ; Mohamed, Norani Muti ^{[1, 5]} ; Joshi, Nirav ^{[6, 7]}

Affiliation

^[1] Univ Teknol PETRONAS, Ctr Innovat Nanostruct & Nanodevices COINN, Seri Iskandar 32610, Perak Darul Rid - Malaysia

^[2] Univ Teknol PETRONAS, Dept Mech Engn, Seri Iskandar, Perak Darul Rid - Malaysia

^[3] Univ Malaysia Perlis, Inst Nano Elect Engn, Kangar, Perlis - Malaysia

^[4] Univ Malaysia Perlis, Sch Bioproc Engn, Arau, Perlis - Malaysia

^[5] Univ Teknol PETRONAS, Dept Fundamental & Appl Sci, Seri Iskandar, Perak Darul Rid - Malaysia

^[6] Univ Calif Berkeley, Dept Mech Engn, Berkeley, CA 94720 - USA

^[7] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Biotechnology and Applied Biochemistry; v. 68, n. 6, p. 1386-1395, DEC 2021.
• Web of Science Citations: 6

Abstract

Acute myocardial infarction (AMI) is one of the leading causes of death worldwide. Cardiac troponin I (cTn1) is a commonly used biomarker for the diagnosis of AMI. Although there are various detection methods for the rapid detection of cTn1 such as optical, electrochemical, and acoustic techniques, electrochemical aptasensing techniques are commonly used because of their ease of handling, portability, and compactness. In this study, an electrochemical cTn1 biosensor, MoS2 nanoflowers on screen-printed electrodes assisted by aptamer, was synthesized using hydrothermal technique. Field emission scanning electron microscopy revealed distinct 2D nanosheets and jagged flower-like 3D MoS2 nanoflower structure, with X-ray diffraction analysis revealing well-stacked MoS(2 )layers. Voltammetry aptasensing of cTn1 ranges from 10 fM to 1 nM, with a detection limit at 10 fM and a sensitivity of 0.10 nA mu M-1 cm(-2). This is a similar to fivefold improvement in selectivity compared with the other proteins and human serum. This novel aptasensor retained 90% of its biosensing activity after 6 weeks with a 4.3% RSD and is a promising high-performance biosensor for detecting cTn1. (AU)

• FAPESP's process: 16/23474-6 - Nanomaterial-based heterostructures: synthesis and their gas sensing properties
• Grantee: Niravkumar Jitendrabhai Joshi
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 14/23546-1 - Enhanced sensitive and selective sensors made with p-NiO nanowire/n-ZnO Nanowire heterostructures
• Grantee: Niravkumar Jitendrabhai Joshi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral