Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts

Author(s):Show less -

Gusson-Zanetoni, Juliana Prado ^[1] ; Guaraldi Monteiro da Silva, Julliene Stephanie ^[1] ; Picao, Thais Bravo ^[1] ; Cardin, Laila Toniol ^[1] ; Prates, Janesly ^[1] ; Sousa, Stefanie Oliveira ^[1] ; Henrique, Tiago ^[2] ; Oliani, Sonia Maria ^[1] ; Tajara, Eloiza Helena ^{[2, 3]} ; Andrade E Silva, Marcio Luis ^[4] ; Cunha, Nayanne Larissa ^[4] ; da Silva Gomes, Ana Carolina ^[5] ; Laurentiz, Rosangela Silva ^[5] ; Rodrigues-Lisoni, Flavia Cristina ^{[1, 6]}

Affiliation

^[1] Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci IBILCE, Dept Biol, UNESP, Campus Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP - Brazil

^[2] Sch Med Sao Jose do Rio Preto FAMERP, Dept Mol Biol, Sao Jose Do Rio Preto - Brazil

^[3] Univ Sao Paulo, Inst Biosci, Dept Genet & Evolut Biol, Sao Paulo, SP - Brazil

^[4] Univ Franca UNIFRAN, Nucleus Res Exact & Technol Sci, Franca, SP - Brazil

^[5] Sao Paulo State Univ, Sch Engn FEIS, Dept Phys Chem, UNESP, Campus Ilha Solteira, Ilha Solteira, SP - Brazil

^[6] Sao Paulo State Univ, Sch Engn FEIS, Dept Biol & Anim Sci, UNESP, Campus Ilha Solteira, Ilha Solteira, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHARMACOLOGICAL SCIENCES; v. 148, n. 1, p. 93-102, JAN 2022.
• Web of Science Citations: 0

Abstract

The objective of the present study was to evaluate the action of the crude hydroalcoholic extract of Piper cubeba fruits and isolated lignans (cubebin, dihydrocubebin, ethylcubebin, hinokinin and methylcubebin) on head and neck cancer cells. We evaluated the influence of the Piper cubeba extract and isolated lignans (10, 50 e 100 mg/mL) for 4, 24, 48 and 72 h, in the larynx (Hep-2) and oral (SCC-25) squamous cell carcinoma cells and normal fibroblasts, on morphology, cell proliferation and migration, cytotoxicity, genotoxicity and gene and protein expression (PTGS2, PTGER3, PTGER4, MMP2, MMP9). The results showed that the P. cubeba extract and different lignans do not alter the cellular morphology, but decrease cell proliferation and migration, have low cytotoxic and genotoxic effects, probably due to the alteration of the expression of genes and proteins involved with inflammatory process. From these data, we can conclude that the lignans cubebin and methylcubebin had a greater effect on head and neck cancer cells in the antiproliferative, antimigratory and genotoxic action, and could be the target of the development of new therapies including possible new drugs as a therapeutic resource for the treatment of head and neck cancer due to its immense range of biological properties. (c) 2021 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/ 4.0/). (AU)

• FAPESP's process: 17/02100-3 - Piper cubeba: applications in different tumorigenic cell lines
• Grantee: Flávia Cristina Rodrigues Lisoni
• Support Opportunities: Regular Research Grants