hemometrics applied to H-1 NMR and UV-Vis spectroscopy as a way to evaluate solid-liquid extraction of leaves of artichok

Author(s)

Boffo, Elisangela Fabiana ^[1] ; de Melo, Keture Salles ^[2] ; Shiromoto, Milena Orlof ^[2] ; Silva, Airton Damasceno ^[3] ; Vieira, Paulo Cezar ^[4] ; Pepe Ambrozin, Alessandra Regina ^[2]

Affiliation

^[1] Fed Univ Bahia UFBA, Inst Chem, Dept Organ Chem, Salvador, BA - Brazil

^[2] Fed Univ Alfenas Unifal MG, Inst Sci & Technol, Pocos De Caldas, MG - Brazil

^[3] Fed Univ Sao Carlos UFSCar, Dept Chem, Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Biomol Sci, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 377, MAY 30 2022.
• Web of Science Citations: 0

Abstract

Artichoke has several biological actions, which are related to the synergistic action of its bioactive compounds. Solid-liquid extraction influence the type and quantity of compounds extracted and, consequently, the biological activity of the plant extract. This study aims to investigate which extraction method (maceration, infusion, or Soxhlet) and which green solvent (ethanol or acetone) would be more suitable to obtain bioactive artichoke extracts. All solid-liquid procedures were carried out in triplicate, using 3.0 g of artichoke leaves and 200 mL of solvent. After drying, samples were analyzed by UV-Vis and H-1 NMR. Chemometrics was applied to spectroscopic data, and the PCA analysis showed that they were specially separated according to the solvent extractor. The sesquiterpene lactone cynaropicrin was identified as the major compound of the extracts, and this allowed us to conclude that the best solid-liquid procedure was Soxhlet, using ethanol as solvent. (AU)

• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC