| Full text | |
| Author(s): |
Carvalho, Bruna G.
[1]
;
Garcia, Bianca B. M.
[2]
;
Malfatti-Gasperini, Antonio A.
[3]
;
Han, Sang W.
[2]
;
de la Torre, Lucimara G.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Campinas, Sch Chem Engn, Dept Mat & Bioproc Engn, UNICAMP, BR-13083970 Campinas - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Ctr Cell Therapy & Mol, BR-04044010 Sao Paulo - Brazil
[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | COLLOIDS AND SURFACES B-BIOINTERFACES; v. 210, FEB 2022. |
| Web of Science Citations: | 0 |
| Abstract | |
The association of cationic carriers with different anionic mucoadhesive biopolymers has been widely explored as an alternative to improve their delivery routes and specific targeting. This work presents a complete analysis of the association between chondroitin sulfate (CS) and cationic liposomes (CLs)/lipoplex (CL-pDNA). In this study, plasmid DNA (pDNA) was used as a genetic cargo for association with carriers. Firstly, we measured the stoichiometry of pseudo complexes and evaluated their colloidal properties, structural and morphological characteristics. Optimized CL-pDNA lipoplexes (positive z-potential) and CL-CS / CL-pDNA-CS (negative z-potential with CS mass ratio of 9% (w/w)) were further studied in detail. Small-angle X-ray scattering analysis and cryo-transmission electron microscopy micrographs revealed that the electrostatic interaction between CS and CL / CL-pDNA easily reorganized the lipid bilayers resulting in nanoscale uni/multilamellar vesicles. A high CS mass ratio (9% (w/w)) led to the reassembly of liposomal structure, wherein the pDNA was easily exchanged for CS chains, forming more than 50% of dense multilamellar vesicles. This data evidenced that the association between CS and CLs is not a conventional coating process since it generates complex and hybrid structures. We believe that these obtained colloidal data may be used in the future to investigate polymer-tailored nanocarriers and their production process. In brief, the colloidal study of hybrid structures may open interesting perspectives for developing novel carriers for drug and gene delivery applications. (AU) | |
| FAPESP's process: | 15/20206-8 - Modulation of monocytes, macrophages and pericytes by the colony stimulating factor genes to treat murine limb ischemia |
| Grantee: | Sang Won Han |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/18523-3 - Polymeric microparticle synthesis via droplet microfluidics for sustained release of non-viral vectors applied to gene therapy |
| Grantee: | Bruna Gregatti de Carvalho |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 18/19537-8 - MICROFLUIDICS AS A TECHNOLOGICAL PLATFORM FOR NANO & BIOTECHNOLOGY |
| Grantee: | Lucimara Gaziola de la Torre |
| Support Opportunities: | Regular Research Grants |