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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hybrid polymer/lipid vesicle synthesis: Association between cationic liposomes and lipoplexes with chondroitin sulfate

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Author(s):
Carvalho, Bruna G. [1] ; Garcia, Bianca B. M. [2] ; Malfatti-Gasperini, Antonio A. [3] ; Han, Sang W. [2] ; de la Torre, Lucimara G. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Sch Chem Engn, Dept Mat & Bioproc Engn, UNICAMP, BR-13083970 Campinas - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Ctr Cell Therapy & Mol, BR-04044010 Sao Paulo - Brazil
[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 210, FEB 2022.
Web of Science Citations: 0
Abstract

The association of cationic carriers with different anionic mucoadhesive biopolymers has been widely explored as an alternative to improve their delivery routes and specific targeting. This work presents a complete analysis of the association between chondroitin sulfate (CS) and cationic liposomes (CLs)/lipoplex (CL-pDNA). In this study, plasmid DNA (pDNA) was used as a genetic cargo for association with carriers. Firstly, we measured the stoichiometry of pseudo complexes and evaluated their colloidal properties, structural and morphological characteristics. Optimized CL-pDNA lipoplexes (positive z-potential) and CL-CS / CL-pDNA-CS (negative z-potential with CS mass ratio of 9% (w/w)) were further studied in detail. Small-angle X-ray scattering analysis and cryo-transmission electron microscopy micrographs revealed that the electrostatic interaction between CS and CL / CL-pDNA easily reorganized the lipid bilayers resulting in nanoscale uni/multilamellar vesicles. A high CS mass ratio (9% (w/w)) led to the reassembly of liposomal structure, wherein the pDNA was easily exchanged for CS chains, forming more than 50% of dense multilamellar vesicles. This data evidenced that the association between CS and CLs is not a conventional coating process since it generates complex and hybrid structures. We believe that these obtained colloidal data may be used in the future to investigate polymer-tailored nanocarriers and their production process. In brief, the colloidal study of hybrid structures may open interesting perspectives for developing novel carriers for drug and gene delivery applications. (AU)

FAPESP's process: 15/20206-8 - Modulation of monocytes, macrophages and pericytes by the colony stimulating factor genes to treat murine limb ischemia
Grantee:Sang Won Han
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/18523-3 - Polymeric microparticle synthesis via droplet microfluidics for sustained release of non-viral vectors applied to gene therapy
Grantee:Bruna Gregatti de Carvalho
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/19537-8 - MICROFLUIDICS AS A TECHNOLOGICAL PLATFORM FOR NANO & BIOTECHNOLOGY
Grantee:Lucimara Gaziola de la Torre
Support Opportunities: Regular Research Grants