Interaction of periodontitis and orthodontic tooth movement-an in vitro and in vivo study

Author(s)

Rath-Deschner, Birgit ^[1] ; Nogueira, Andressa V. B. ^[2] ; Beisel-Memmert, Svenja ^[1] ; Nokhbehsaim, Marjan ^[3] ; Eick, Sigrun ^[4] ; Cirelli, Joni A. ^[5] ; Deschner, James ^[2] ; Jaeger, Andreas ^[1] ; Damanaki, Anna ^[2]

Affiliation

^[1] Univ Bonn, Ctr Dentomaxillofacial Med, Dept Orthodont, Welschnonnenstr 17, D-53111 Bonn - Germany

^[2] Johannes Gutenberg Univ Mainz, Dept Periodontol & Operat Dent, Univ Med Ctr, Mainz - Germany

^[3] Univ Bonn, Ctr Dentomaxillofacial Med, Sect Expt Dentomaxillofacial Med, Bonn - Germany

^[4] Univ Bern, Dept Periodontol, Lab Oral Microbiol, Bern - Switzerland

^[5] Sao Paulo State Univ, Sch Dent Araraquara, Dept Diag & Surg, UNESP, Araraquara, SP - Brazil

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 26, n. 1, p. 171-181, JAN 2022.
• Web of Science Citations: 1

Abstract

Objectives The aim of this in vitro and in vivo study was to investigate the interaction of periodontitis and orthodontic tooth movement on interleukin (IL)-6 and C-X-C motif chemokine 2 (CXCL2). Materials and methods The effect of periodontitis and/or orthodontic tooth movement (OTM) on alveolar bone and gingival IL-6 and CXCL2 expressions was studied in rats by histology and RT-PCR, respectively. The animals were assigned to four groups (control, periodontitis, OTM, and combination of periodontitis and OTM). The IL-6 and CXCL2 levels were also studied in human gingival biopsies from periodontally healthy and periodontitis subjects by RT-PCR and immunohistochemistry. Additionally, the synthesis of IL-6 and CXCL2 in response to the periodontopathogen Fusobacterium nucleatum and/or mechanical strain was studied in periodontal fibroblasts by RT-PCR and ELISA. Results Periodontitis caused an increase in gingival levels of IL-6 and CXCL2 in the animal model. Moreover, orthodontic tooth movement further enhanced the bacteria-induced periodontal destruction and gingival IL-6 gene expression. Elevated IL-6 and CXCL2 gingival levels were also found in human periodontitis. Furthermore, mechanical strain increased the stimulatory effect of F. nucleatum on IL-6 protein in vitro. Conclusions Our study suggests that orthodontic tooth movement can enhance bacteria-induced periodontal inflammation and thus destruction and that IL-6 may play a pivotal role in this process. (AU)

• FAPESP's process: 14/20715-7 - INFLUENCE OF ORTHODONTIC TOOTH MOVEMENT ON PERIODONTAL DISEASE PROGRESSION IN NORMAL SYSTEMIC HEALTH OR IN OBESITY
• Grantee: Andressa Vilas Boas Nogueira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/07137-2 - Effects of obesity on periodontal tissues subjected to biomechanical forces
• Grantee: Joni Augusto Cirelli
• Support Opportunities: Regular Research Grants