omparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importanc

Author(s):Show less -

Lara, Bruna Rossini ^{[1, 2]} ; de Camargo, Bruno Braidotti ^[2] ; Paula, Claudete Rodrigues ^[3] ; Leite Junior, Diniz Pereira ^[4] ; Garces, Hans Garcia ^[1] ; Arnoni, Mariana Volpe ^{[5, 6]} ; Silveira, Monica ^[7] ; Favero Gimenes, Viviane Mazo ^[8] ; Machado Siqueira, Lumena Pereira ^[8] ; Fernandes Takahashi, Juliana Possatto ^[9] ; Carvalho Melhem, Marcia de Souza ^[9] ; Richini-Pereira, Virginia Bodelao ^[2] ; Anversa, Lais ^[2] ; Ruiz, Luciana da Silva ^[2]

Affiliation

^[1] Sao Paulo State Univ Unesp, Inst Biosci, BR-18618689 Botucatu, SP - Brazil

^[2] Biomed Sci Ctr, Adolfo Lutz Inst IAL CLR II, BR-17015110 Bauru, SP - Brazil

^[3] Univ Sao Paulo, Sch Dent, BR-05508000 Sao Paulo, SP - Brazil

^[4] Fed Univ Mato Grosso UFMT, Fac Med, Av Fernando Correa Costa, BR-2367 Cuiaba, MT - Brazil

^[5] Irmandade Santa Casa Misericordia Sao Paulo, BR-01221010 Sao Paulo, SP - Brazil

^[6] Darcy Vargas Childrens Hosp, BR-05614040 Sao Paulo, SP - Brazil

^[7] Bauru State Hosp HEB, BR-17033360 Bauru, SP - Brazil

^[8] Univ Sao Paulo, Inst Trop Med, BR-05403000 Sao Paulo, SP - Brazil

^[9] Adolfo Lutz Cent Inst IAL, Mycol Sect, BR-01246000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Medical Mycology; v. 59, n. 12, p. 1181-1190, DEC 2021.
• Web of Science Citations: 0

Abstract

Trichosporon spp. are widely distributed in the nature, comprising species that inhabit different ecological niches and can be found in the water, soil, and body surface of animals and humans. Such microorganisms have been classically associated with superficial infections; however, in the last decades, they have also been related to disseminated infections in immunocompromised patients, behaving as opportunistic agents, which demands rapid and accurate species identification for efficient therapy. Concordance level between the traditional phenotypic method and the molecular technique (gold standard) in the identification of all 59 Trichosporon samples was 59.3%. Identification concordance between MALDI-TOF spectrometry and the molecular technique was 71.2%. No isolate of environmental origin was identifiable by MALDI-TOF mass spectrometry (MS), and 100% of such environmental isolates were discordant for IGS region sequencing and phenotypic characterization. Both comparisons evidenced greatest concordance in the identification of T. asahii. The species T. debeurmannianum, T. dermatis, T. venhuisii and T. insectorum were not properly identified by both MALDI-TOF MS and the phenotypic technique. MALDI-TOF MS, in particular, seems to be appropriate to investigate yeasts of the genus Trichosporon; however, database updates are still necessary, especially for species that are not common in the clinical routine. With the aim of helping understand the aspects involved in early and accurate diagnosis of infections caused by this opportunistic agent, the present study compared the phenotypic, molecular (IGS region) and mass-spectrometry (MALDI-TOF) identification of 59 yeasts of the genus Trichosporon which had clinical and environmental origin and were kept in a mycology collection. (AU)

• FAPESP's process: 19/08642-8 - Clinical and environmental isolates of Trichosporon spp.: characterization, virulence research and profile of susceptibility to antifungals
• Grantee: Luciana da Silva Ruiz Menezes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/08419-7 - Clinical and environmental isolates of Trichosporon spp.: characterization, virulence research and profile of susceptibility to antifungals
• Grantee: Bruna Rossini Lara
• Support Opportunities: Scholarships in Brazil - Master