idgut and fat body: Multisystemic action of pyriproxyfen on non-target organism Ceraeochrysa claveri (Neuroptera: Chrysopidae

Author(s)

Scudeler, Elton Luiz ^[1] ; de Carvalho, Shelly Favorito ^[2] ; Gimenes Garcia, Ana Silvia ^[1] ; Santorum, Marilucia ^[1] ; Padovani, Carlos Roberto ^[3] ; dos Santos, Daniela Carvalho ^{[1, 2]}

Affiliation

^[1] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, Lab Insects, UNESP, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Electron Microscopy Ctr, Inst Biosci Botucatu, UNESP, Botucatu, SP - Brazil

^[3] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Biostat, UNESP, Botucatu, SP - Brazil

• Document type: Journal article
• Source: Environmental Pollution; v. 293, JAN 15 2022.
• Web of Science Citations: 0

Abstract

Morphological tools can assist in the evaluation of effects of insecticides on non-target insects. Pyriproxyfen, a juvenile hormone analog, is known to interfere with growth and metamorphosis of insects. However, there are studies showing indirect effects on natural enemies, including green lacewings. Few prior studies describe morphological effects of pyriproxyfen on target insect organs, especially on natural enemies. Through morphological tools, this study aimed to characterize the midgut and fat body, both important organs of digestion and great metabolic activity respectively, of the predator Ceraeochrysa claveri after chronic exposure to pyriproxyfen. Larvae of C. claveri were fed Diatraea saccharalis egg clusters treated with pyriproxyfen in solution of 50 or 100 mg a.i. L-1 throughout the larval stage. The biological data revealed significant increases in development time, especially in the third instar, and in cumulative mortality from the prepupal into the pupal stage. Morphological analysis of adult midgut (<= 24 h old) showed damage including formation of epithelial folds, intercellular spaces, emission of cytoplasmic protrusions. Both fat body regions presented decrease of lipid droplets, vacuolization of trophocytes and mitochondrial injury featuring a multisystemic action. In both organs, pyriproxyfen exposure induced significant oxidative stress by mitochondrial superoxide production. Cytoprotective responses were induced in midgut and fat body cells by augmenting the number of cytoplasmic granules containing calcium and expression of HSP 90. Both organs proved to be efficient in presenting histopathological alterations, showing the sensitivity and applicability of this morphological tool for evaluating other insecticides in non-target organisms. (AU)

• FAPESP's process: 17/15120-2 - Comparative analysis of the effects of the biopesticide Azadirachtin and the synthetic insecticide Piriproxifem on the testes of Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae): a morphological, ultrastructural and molecular approach.
• Grantee: Daniela Carvalho dos Santos
• Support Opportunities: Regular Research Grants