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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of systemic inflammation due to hepatic ischemia-reperfusion injury upon lean or obese visceral adipose tissue

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Author(s):
Ligia Fernanda Ferraz [1] ; Cintia Rabelo e Paiva Caria [2] ; Raquel de Cássia Santos [3] ; Marcelo Lima Ribeiro [4] ; Alessandra Gambero [5]
Total Authors: 5
Affiliation:
[1] Universidade São Francisco. Postgraduate Program in Health Science - Brasil
[2] Universidade Estadual de Campinas. School of Food Engineering. Department of Food and Nutrition - Brasil
[3] Universidade São Francisco. Postgraduate Program in Health Science - Brasil
[4] Universidade São Francisco. Postgraduate Program in Health Science - Brasil
[5] Pontifícia Universidade Católica de Campinas. Life Science Center - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Acta Cirurgica Brasileira; v. 37, n. 1 2022-03-14.
Abstract

ABSTRACT Purpose: To evaluate how the induction of liver damage by ischemia and reperfusion affects the adipose tissue of lean and obese mice. Methods: Lean and diet-induced obese mice were subjected to liver ischemia (30 min) followed by 6 h of reperfusion. The vascular stromal fraction of visceral adipose tissue was analyzed by cytometry, and gene expression was evaluated by an Array assay and by RT-qPCR. Intestinal permeability was assessed by oral administration of fluorescein isothiocyanate (FITC)-dextran and endotoxemia by serum endotoxin measurements using a limulus amebocyte lysate assay. Results: It was found that, after liver ischemia and reperfusion, there is an infiltration of neutrophils, monocytes, and lymphocytes, as well as an increase in the gene expression that encode cytokines, chemokines and their receptors in the visceral adipose tissue of lean mice. This inflammatory response was associated with the presence of endotoxemia in lean mice. However, these changes were not observed in the visceral adipose tissue of obese mice. Conclusions: Liver ischemia and reperfusion induce an acute inflammatory response in adipose tissue of lean mice characterized by an intense chemokine induction and leukocyte infiltration; however, inflammatory alterations are already present at baseline in the obese adipose tissue and liver ischemia and reperfusion do not injure further. (AU)

FAPESP's process: 16/17640-0 - Effect of systemic inflammation following ischemia / reperfusion upon the visceral adipose tissue of mice
Grantee:Lígia Fernanda Ferraz
Support Opportunities: Scholarships in Brazil - Master