The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development

Author(s)

Braga, Aline Silva ; Abdelbary, Mohamed Mostafa Hefny ; Kim, Rafaela Ricci ; de Souza Rosa de Melo, Fernanda Pereira ; Saldanha, Luiz Leonardo ; Dokkedal, Anne Ligia ; Conrads, Georg ; Esteves-Oliveira, Marcella ; Magalhaes, Ana Carolina

• Document type: Journal article
• Source: ANTIBIOTICS-BASEL; v. 11, n. 3, p. 14-pg., 2022-03-01.

Abstract

This study investigated the effects of herbal toothpaste on bacterial counts and enamel demineralization. Thirty-six bovine enamel samples were exposed to a microcosm biofilm using human saliva and McBain saliva (0.2% sucrose) for 5 days at 37 degrees C and first incubated anaerobically, then aerobically-capnophilically. The following experimental toothpaste slurries (2 x 2 min/day) were applied: (1) Vochysia tucanorum (10 mg/g); (2) Myrcia bella (5 mg/g); (3) Matricaria chamomilla (80 mg/g); (4) Myrrha and propolis toothpaste (commercial); (5) fluoride (F) and triclosan (1450 ppm F), 0.3% triclosan and sorbitol (Colgate(R), positive control); (6) placebo (negative control). The pH of the medium was measured, bacteria were analyzed using quantitative polymerase chain reaction, and enamel demineralization was quantified using transverse microradiography. The total bacterial count was reduced by toothpaste containing Myrcia bella, Matricaria chamomilla, fluoride, and triclosan (commercial) compared to the placebo. As far as assessable, Myrcia bella, Matricaria chamomilla, and Myrrha and propolis (commercial) inhibited the outgrowth of S. mutans, while Lactobacillus spp. were reduced/eliminated by all toothpastes except Vochysia tucanorum. Mineral loss and lesion depth were significantly reduced by all toothpastes (total: 1423.6 +/- 115.2 vol% x mu m; 57.3 +/- 9.8 mu m) compared to the placebo (2420.0 +/- 626.0 vol% x mu m; 108.9 +/- 21.17 mu m). Herbal toothpastes were able to reduce enamel demineralization. (AU)

• FAPESP's process: 17/00556-0 - Evaluation of the antimicrobial effect of alternative and commercial agents on a microcosm biofilm and on prevention of dental demineralization
• Grantee: Ana Carolina Magalhães
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/17249-2 - Evaluation of the effect of different experimental and commercial natural extracts in the form of toothpastes and oral mouthrinses on the viability and activity of microcosm biofilm and on enamel demineralization prevention in vitro
• Grantee: Aline Silva Braga
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/21797-0 - Effect of artificial saliva substitutes and fluoride application on the development of root caries under a microcosm biofilm model simulating patients subjected to head and neck radiation
• Grantee: Ana Carolina Magalhães
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/26506-1 - Effect of different experimental toothpastes and oral mouthrinses containing natural extracts on the relative metabolic activity of microcosm biofilm and on enamel demineralization prevention in vitro
• Grantee: Aline Silva Braga
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate