Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight

Author(s)

Gebim Polizel, Guilherme Henrique ; Correia Queiroz Cancado, Fernando Augusto ; Ferreira Dias, Evandro Fernando ; Fernandes, Aricia Christofaro ; Cracco, Roberta Cavalcante ; Carmona, Brenda Tonsic ; Castellar, Henrique Hespanhol ; Poleti, Mirele Daiana ; de Almeida Santana, Miguel Henrique

• Document type: Journal article
• Source: METABOLITES; v. 12, n. 5, p. 13-pg., 2022-05-01.

Abstract

This study investigated the effect of prenatal nutrition on liver metabolome and on body (BW) and liver weight (LW) of Nellore bulls at slaughter. Three treatments were applied in 126 cows during pregnancy: NP-control (mineral supplementation); PP-protein-energy supplementation in the third trimester; and FP-protein-energy supplementation during the entire pregnancy. Offspring BW and LW were evaluated, and a targeted metabolomics analysis was performed on their livers (n = 18, 22.5 +/- 1 months of age). Data were submitted to principal component analysis (PCA), analysis of variance (ANOVA), enrichment analysis, and Pearson's correlation analysis. The phenotypes did not show differences between treatments (p > 0.05). Metabolites PCA showed an overlap of treatment clusters in the analysis. We found significant metabolites in ANOVA (p <= 0.05; Glycine, Hydroxytetradecadienylcarnitine, Aminoadipic acid and Carnosine). Enrichment analysis revealed some biological processes (Histidine metabolism, beta-Alanine metabolism, and Lysine degradation). Pearson's correlation analysis showed 29 significant correlated metabolites with BW and 1 metabolite correlated with LW. In summary, prenatal nutrition did not show effects on the phenotypes evaluated, but affected some metabolites and biological pathways, mainly related to oxidative metabolism. In addition, BW seems to influence the hepatic metabolome more than LW, due to the amount and magnitude of correlations found. (AU)

• FAPESP's process: 17/12105-2 - Fenomics of fetal programming effects on beef cattle production: a systemic approach
• Grantee: Miguel Henrique de Almeida Santana
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 21/03265-1 - Use of systems biology tools to evaluate the effects of fetal programming in beef cattle
• Grantee: Guilherme Henrique Gebim Polizel
• Support Opportunities: Scholarships in Brazil - Doctorate