Effects of Low-Intensity and Long-Term Aerobic Exercise on the Psoas Muscle of mdx Mice: An Experimental Model of Duchenne Muscular Dystrophy

Author(s)

Sigoli, Emilly ; Antao, Rosangela Aline ; Guerreiro, Maria Paula ; Passos de Araujo, Tatiana Oliveira ; dos Santos, Patty Karina ; da Roza, Daiane Leite ; Rassier, Dilson E. ; Cornachione, Anabelle Silva

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 23, n. 9, p. 15-pg., 2022-05-01.

Abstract

Duchenne muscular dystrophy (DMD) is a muscle disease characterized by the absence of the protein dystrophin, which causes a loss of sarcolemma integrity, determining recurrent muscle injuries, decrease in muscle function, and progressive degeneration. Currently, there is a need for therapeutic treatments to improve the quality of life of DMD patients. Here, we investigated the effects of a low-intensity aerobic training (37 sessions) on satellite cells, peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1 alpha protein (PGC-1 alpha), and different types of fibers of the psoas muscle from mdx mice (DMD experimental model). Wildtype and mdx mice were randomly divided into sedentary and trained groups (n = 24). Trained animals were subjected to 37 sessions of low-intensity running on a motorized treadmill. Subsequently, the psoas muscle was excised and analyzed by immunofluorescence for dystrophin, satellite cells, myosin heavy chain (MHC), and PGC-1 alpha content. The minimal Feret's diameters of the fibers were measured, and light microscopy was applied to observe general morphological features of the muscles. The training (37 sessions) improved morphological features in muscles from mdx mice and caused an increase in the number of quiescent/activated satellite cells. It also increased the content of PGC-1 alpha in the mdx group. We concluded that low-intensity aerobic exercise (37 sessions) was able to reverse deleterious changes determined by DMD. (AU)

• FAPESP's process: 13/07104-6 - Effects of eccentric exercise in skeletal muscle rehabilitation of mdx mice after long period of training: morphological, functional and molecular evaluations
• Grantee: Anabelle Silva Cornachione
• Support Opportunities: Research Grants - Young Investigators Grants