Karyotype description and comparative chromosomal mapping of rDNA and U2 snDNA sequences in Eigenmannia limbata and E. microstoma (Teleostei, Gymnotiformes, Sternopygidae)

Author(s)

Andres Araya-Jaime, Cristian ; Zerbinato de Andrade Silva, Duilio Mazzoni ; Ribeiro da Silva, Luis Ricardo ; Neves do Nascimento, Cristiano ; Oliveira, Claudio ; Foresti, Fausto

• Document type: Journal article
• Source: COMPARATIVE CYTOGENETICS; v. 16, n. 2, p. 16-pg., 2022-05-05.

Abstract

The genus Eigenmannia Jordan et Evermann,1896 includes electric fishes endemic to the Neotropical region with extensive karyotype variability and occurrence of different sex chromosome systems, however, cytogenetic studies within this group are restricted to few species. Here, we describe the karyotypes of Eigenmannia limbata (Schreiner et Miranda Ribeiro, 1903) and E. microstoma (Reinhardt, 1852) and the chromosomal locations of 5S and 18S rDNAs (ribosomal RNA genes) and U2 snDNA (small nuclear RNA gene). Among them, 18S rDNA sites were situated in only one chromosomal pair in both species, and co-localized with 5S rDNA in E. microstoma. On the other hand, 5S rDNA and U2 snRNA sites were observed on several chromosomes, with variation in the number of sites between species under study. These two repetitive DNAs were observed co-localized in one chromosomal pair in E. limbata and in four pairs in E. microstoma. Our study shows a new case of association of these two types of repetitive DNA in the genome of Gymnotiformes. (AU)

• FAPESP's process: 17/15608-5 - Meiotic chromosomes in fish: synaptic behavior and transcriptional status visualized by immunofluorescence.
• Grantee: Fausto Foresti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/22447-8 - Comparative Genetic Activity on B Chromosomes of Astyanax species (Teleostei, Characiformes)
• Grantee: Duílio Mazzoni Zerbinato de Andrade Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/09634-5 - Functional and evolutionary analysis of b chromosomes using the Astyanax paranae fish (Characiformes Characidae) as model
• Grantee: Fausto Foresti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/09204-6 - Biodiversity and Biogeography in marine-estuarine fishes (Teleostei) from Brazil
• Grantee: Claudio de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/26508-3 - Phylogeny of the hyperdiverse order Characiformes (Teleostei: Ostariophysi) using ultraconserved elements
• Grantee: Claudio de Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/20610-1 - Study of the biogeography of the Brazilian coast using data population genetic data of marine catfish of the genera Cathorops and Genidens
• Grantee: Claudio de Oliveira
• Support Opportunities: Regular Research Grants