Whole-genome analysis of haemophilus influenzae invasive strains isolated from Campinas state University hospital. An epidemiological approach 2012-2019 and ancestor strains

Author(s)

Pereira, Rafaella Fabiana Carneiro ; Guarnieri, Joao Paulo de Oliveira ; da Silva, Carlos Fernando Macedo ; Bernardes, Bruno Gaia ; Lancellotti, Marcelo

• Document type: Journal article
• Source: Brazilian Journal of Infectious Diseases; v. 26, n. 1, p. 4-pg., 2022-01-01.

Abstract

Thirteen Haemophylus influenzae invasive strains isolated from patients at Clinical Hospital of State University of Campinas, from May 2013 through August 2019, was submitted to Illumina genome sequencing HiSeq platform. Further in silico analysis of serogroup and Multi Locus Sequence Typing (MLST) from whole DNA sequencing had demonstrated the actual clonal distribution in the Campinas Metropolitan region. Thus, results showed the existence of a new ST Haemophilus influenzae found in the Brazilian territory and an increase of strains belonging to serogroup a (three strains also belonging to ST23). In conclusion, we observed an increase of non-typable H. influenzae (NTHi) and a strain involved in invasive diseases in the Campinas - Sa~o Paulo region after frequent detection of those serotypes and genotypes in other Brazilian regions.(c) 2021 Sociedade Brasileira de Infectologia. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) (AU)

• FAPESP's process: 18/09874-7 - Analysis and use of the genome of Haemophilus influenzae biotype aegyptius for biotechnologies purposes
• Grantee: Marcelo Lancellotti
• Support Opportunities: Regular Research Grants