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Analysis and use of the genome of Haemophilus influenzae biotype aegyptius for biotechnologies purposes

Grant number: 18/09874-7
Support Opportunities:Regular Research Grants
Start date: November 01, 2018
End date: October 31, 2020
Field of knowledge:Health Sciences - Pharmacy
Principal Investigator:Marcelo Lancellotti
Grantee:Marcelo Lancellotti
Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

This project aims to understand the virulence factors of Haemophilus influenzae biotype aegyptius through techniques of transfer of fused genes of this microorganism to strains of this bacterial species that do not cause this clinical picture. In this way, experiments of fusion of genes of interest to antibiotic resistance cassettes and subsequent verification of the transfer of the resistance together with the virulence characteristics of the microorganisms. Initially, we will use genes that the group is already working and seem to be involved with such a pathological process linked to the virulence of this microorganism (crgA, hadA, las and tabA). An approach involving the mutations of these genes in wild strains has already been attempted and was not positive due to the loss of the recombination capacity of the strains involved in the pathophysiological processes of purple fever in the 80's. In addition, due to the isolation of new strains of interest epidemiological and microbiological aspects of H. influenzae bacteria in invasive disease episodes in 2015, we propose the genomic analysis of some of these strains to deepen the knowledge about their virulence and similarities with the Hae-BPF strains. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREIRA, RAFAELLA FABIANA CARNEIRO; GUARNIERI, JOAO PAULO DE OLIVEIRA; DA SILVA, CARLOS FERNANDO MACEDO; BERNARDES, BRUNO GAIA; LANCELLOTTI, MARCELO. Whole-genome analysis of haemophilus influenzae invasive strains isolated from Campinas state University hospital. An epidemiological approach 2012-2019 and ancestor strains. Brazilian Journal of Infectious Diseases, v. 26, n. 1, p. 4-pg., . (18/09874-7)
JOSÉ DIOGO DE OLIVEIRA; CARLOS FERNANDO MACEDO; JOÃO PAULO DE OLIVEIRA GUARNIERI; THAÍS HOLTZ THEIZEN; DAISY MACHADO; MARCELO LANCELLOTTI. Influence of environmental dust in transformation capacity of Streptococcus pneumoniae. GENETICS AND MOLECULAR BIOLOGY, v. 44, n. 1, . (12/15046-3, 18/09874-7, 11/14079-2)
RAFAELLA FABIANA CARNEIRO PEREIRA; THAIS HOLTZ THEIZEN; DAISY MACHADO; JOÃO PAULO DE OLIVEIRA GUARNIERI; GABRIEL PICCIRILLO GOMIDE; LUCIANA MARIA DE HOLLANDA; MARCELO LANCELLOTTI. Analysis of potential virulence genes and competence to transformation in Haemophilus influenzae biotype aegyptius associated with Brazilian Purpuric Fever. GENETICS AND MOLECULAR BIOLOGY, v. 44, n. 1, . (18/09874-7, 13/00847-3, 11/01319-5, 18/07808-7, 12/15046-3)