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Analysis and use of the genome of Haemophilus influenzae biotype aegyptius for biotechnologies purposes

Grant number: 18/09874-7
Support type:Regular Research Grants
Duration: November 01, 2018 - October 31, 2020
Field of knowledge:Health Sciences - Pharmacy
Principal Investigator:Marcelo Lancellotti
Grantee:Marcelo Lancellotti
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

This project aims to understand the virulence factors of Haemophilus influenzae biotype aegyptius through techniques of transfer of fused genes of this microorganism to strains of this bacterial species that do not cause this clinical picture. In this way, experiments of fusion of genes of interest to antibiotic resistance cassettes and subsequent verification of the transfer of the resistance together with the virulence characteristics of the microorganisms. Initially, we will use genes that the group is already working and seem to be involved with such a pathological process linked to the virulence of this microorganism (crgA, hadA, las and tabA). An approach involving the mutations of these genes in wild strains has already been attempted and was not positive due to the loss of the recombination capacity of the strains involved in the pathophysiological processes of purple fever in the 80's. In addition, due to the isolation of new strains of interest epidemiological and microbiological aspects of H. influenzae bacteria in invasive disease episodes in 2015, we propose the genomic analysis of some of these strains to deepen the knowledge about their virulence and similarities with the Hae-BPF strains. (AU)