Basiliximab Does Not Impair Airway Mucociliary Clearance of Rats

Author(s):Show less -

Correia, Aristides Tadeu ; de Almeida, Francine Maria ; Augusto-Cottet, Marcia Cristina ; Nolasco, Patricia ; Alves Bento, Afonso Silva ; Matsushima Hirano, Hugo Kenji ; Ribeiro de Souza, Maria Cecilia ; dos Santos, Elizabete Silva ; Rodrigues de Castro, Julia Helena ; Matsuda, Monique ; Pego-Fernandes, Paulo Manuel ; Pazetti, Rogerio

• Document type: Journal article
• Source: Inflammation; v. N/A, p. 13-pg., 2022-06-17.

Abstract

Previous studies have shown that immunosuppressive drugs impair the airway mucociliary clearance of rats. However, considering the high specificity of basiliximab (BSX) and the absence of studies reporting its side effects, our aim was to investigate whether BSX, associated or not with triple therapy, impairs the mucociliary system. Forty rats were divided into 4 groups: Control, BSX, Triple, and BSX + Triple. After 15 days of treatment, animals were euthanized and the ciliary beating frequency (CBF), mucociliary transport velocity (MCTV), neutral and acid mucin production, Muc5ac and Muc5b gene expression, inflammatory cell number, and interleukin (IL)-6 concentration were analyzed. CBF and MCTV were lower in Triple and BSX + Triple groups (p < 0.05). Neutral mucin percentage was higher in Triple group (p < 0.05), and acid mucin percentage was higher in Triple and BSX + Triple groups (p < 0.05). The Muc5ac and Muc5b gene expression was higher in Triple and BSX + Triple groups (p < 0.05). Animals from Triple and BSX + Triple groups presented fewer mononuclear cells (p < 0.05). The number of polymorphonuclear cells was higher in the Triple group (p < 0.05). In the analysis of inflammatory cells in the blood, there was a decrease in lymphocytes and an increase in neutrophils in the Triple and BSX + Triple groups (p < 0.05). The concentration of IL-6 significantly increased in the animals of the Triple and BSX + Triple groups (p < 0.05). BSX did not change the mucociliary apparatus of rats. (AU)

• FAPESP's process: 12/09839-0 - Effects of basiliximab with and without triple therapy on mucociliary system of rats airway: experimental study
• Grantee: Rogerio Pazetti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/17708-0 - XVII Congresso Luso Brasileiro de Transplantação
• Grantee: Aristides Tadeu Correia
• Support Opportunities: Research Grants - Meeting - Abroad
• FAPESP's process: 14/17525-1 - Effects of basiliximab with and without triple therapy on airway mucociliary clearance of rats: experimental study
• Grantee: Maria Cecília Ribeiro de Souza
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/17924-4 - Effects of basiliximab upon airway mucus production of rats
• Grantee: Júlia Helena Rodrigues de Castro
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/24085-2 - Effects of basiliximab associated or not with triple therapy on airway mucociliary clearance of rats: experimental study
• Grantee: Hugo Kenji Matsushima Hirano
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/18784-5 - Effects of basiliximab associated or not with triple therapy on airway mucociliary clearance of rats: experimental study
• Grantee: Afonso da Silva Alves Bento
• Support Opportunities: Scholarships in Brazil - Scientific Initiation