Effects of basiliximab with and without triple therapy on airway mucociliary clearance of rats: experimental study

• Grant number: 14/17525-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2015
• Effective date (End): December 31, 2015
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Rogerio Pazetti
• Grantee: Maria Cecília Ribeiro de Souza
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

To complement the immunosuppression regimens, at the time the lung transplantation or early postoperative period many centers are using basiliximab as induction therapy which is an antibody antagonist of Interleukin-2 receptor. It is known that infection is the main cause of death in the first month after lung transplantation, coinciding with the same period of greater dysfunction of mucociliary epithelium. That acute dysfunction disables the mucociliary apparatus to protect and purify the air, increasing the possibility of infection. Because of the specificity of basiliximab and a mild adverse effect profile, we believe that this drug do not have any effect on mucociliary system. To test this hypothesis, we will use 120 male rats. The animals will be divided into four groups, each one with three subgroups, based upon the date of euthanasia (7, 15 or 30 days): Group 1: saline; Group 2: basiliximab; Group 3: tacrolimus, mycophenolate sodium and prednisone; and Group 4: tacrolimus, mycophenolate sodium, prednisone and basiliximab. Ciliary beat frequency, mucociliary transport velocity, histology and morphometry of airways will be evaluated. In addition, qualitative and quantitative analysis of mRNA expression for gene Muc5ac goblet cells by PCR real time and quantification of the mucin from Muc5ac gene by Western blotting will be performed.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORREIA, ARISTIDES TADEU; DE ALMEIDA, FRANCINE MARIA; AUGUSTO-COTTET, MARCIA CRISTINA; NOLASCO, PATRICIA; ALVES BENTO, AFONSO SILVA; MATSUSHIMA HIRANO, HUGO KENJI; RIBEIRO DE SOUZA, MARIA CECILIA; DOS SANTOS, ELIZABETE SILVA; RODRIGUES DE CASTRO, JULIA HELENA; MATSUDA, MONIQUE; et al. Basiliximab Does Not Impair Airway Mucociliary Clearance of Rats. Inflammation, v. N/A, p. 13-pg., 2022-06-17. (12/09839-0, 18/17708-0, 14/17525-1, 18/17924-4, 12/24085-2, 12/18784-5)