Effects of hybrid inorganic-organic nanofibers on the properties of enamel resin infiltrants - An in vitro study

Author(s)

Obeid, Alyssa Teixeira ; Antunes Garcia, Luisa Helena ; de Lima Nascimento, Tatiana Rita ; Cancado Castellano, Lucio Roberto ; Soares Bombonatti, Juliana Fraga ; Honorio, Heitor Marques ; Lia Mondelli, Rafael Francisco ; Sauro, Salvatore ; de Amoedo Campos Velo, Marilia Mattar

• Document type: Journal article
• Source: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 126, p. 10-pg., 2022-02-01.

Abstract

This in vitro study aimed to evaluate the overall mechanical properties of resin infiltrants doped with bioactive nanofibers and their ability in inhibiting enamel demineralization or achieving remineralization of the adjacent enamel to white spots. A commercial resin infiltrant (ICON, DMG) was doped with hybrid inorganic-organic nanofibers and analyzed for degree of conversion (DC, n = 3) and surface hardness (SH, n = 6). Subsequently, enamel specimens (6 x 4 x 2 mm(3)) were prepared and submitted to a demineralizing/remineralizing process to produce a subsurface caries-like lesion. The specimens were treated with one of the following materials: ICON infiltrant, DMG (control); ICON + nanofibers of poly-lactic acid (PLA)-filled with silica (PLA-SiO2); ICON + nanofibers of (PLA)-filled with calcium incorporated into a silica network (SiO2-CaP). Then, the specimens were subjected to a pH-cycling demineralizing/remineralizing model for 7 days at 37 ?. The %delta SH change (after treatment), %SH loss and %SH recovery (after pH-cycling regimen) were calculated after SH evaluation (n = 9/group). The Ca/P weight ratio before and after pH-cycling regimen was evaluated through SEM/EDX. The results of DC were analyzed through the T-test (p < 0.05). ANOVA followed by Tukey's test (p < 0.05) was performed for hardness and EDX. A significant SH increase was observed in the ICON/SiO2CaP group (p < 0.05). The ICON/PLA-SiO2 presented higher DC values than the control group (p = 0.043). All groups presented significant difference in %delta SH (p < 0.05), although the specimens treated with ICON/SiO2CaP presented greater values. Regarding the %SHL and %SHR, the ICON/SiO2CaP and ICON/PLA-SiO2 were significantly different compared to the control group (p < 0.001). However, no difference was observed between the ICON/ SiO2CaP and ICON/PLA-SiO2. The Ca/P ratio showed that the ICON/SiO2CaP and ICON/PLA-SiO2 after the pH cycling regimen differed from sound enamel and modified infiltrants before pH-cycling. In conclusion, tailored hybrid nanofibers may be incorporated into enamel resin infiltrants without compromise the mechanical properties of such experimental materials. These latter can inhibit the demineralization of enamel and increase its hardness during pH-clycling challange. (AU)

• FAPESP's process: 19/06045-2 - Synthesis and physical-chemical characterization of a cotton-wool-like nanofibers biopolymer of graphene oxide
• Grantee: Rafael Francisco Lia Mondelli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/10840-2 - Evaluation of resin infiltrant modified by bioactive nanofibers in the treatment of caries-like lesions in bovine enamel
• Grantee: Luísa Helena Antunes Garcia
• Support Opportunities: Scholarships in Brazil - Scientific Initiation