Evaluation of resin infiltrant modified by bioactive nanofibers in the treatment of caries-like lesions in bovine enamel

• Grant number: 19/10840-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2019
• End date: July 31, 2020
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Marilia Mattar de Amoêdo Campos Velo
• Grantee: Luísa Helena Antunes Garcia
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

With the understanding of dental caries as a dynamic process, early detection of the lesions allowed less invasive treatments. Among the strategies for the treatment of active white spot lesions, the resin infiltrant has been highlighted, however, a major limitation of the technique is the inability of the adjacent enamel remineralization, as it only paralyzes caries lesions. Thus, the present research aims to evaluate the anticaries potential of a bioactive resin infiltrant previously developed by this research group. Firstly, 3D hybrid cotton wool-like nanofibers (100 nm) of Poly-lactic acid (PLA) doped or not with bioactive ions were incorporated into a resin infiltrant (ICON, DMG). In this project, bovine tooth enamel blocks (6 × 4 × 2 mm) will be prepared and selected by initial surface hardness. Then, the blocks will be submitted to a demineralizing solution for induction of caries-like lesion and treated with one of the following groups: 1-ICON (control), 2-ICON + PLA/ Ca3(PO4)2 and 3-ICON + PLA/SiO2 (n=12/group). After protection of 1/3 of the exposed surface, a new third will be submitted to a pH-cycling regimen (DES/RE), for 7 days at 37 ° C, in order to evaluate the resistance of the infiltrant in a new cariogenic challenge. The analysis of the variables will be conducted in all conditions (sound, after caries induction, after treatments and after DES/RE challenge), considering surface hardness and X-ray dispersion energy spectroscopy (EDX). The quantitative data will be submitted to two-way ANOVA, followed by Tukey test, with a significance level of 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OBEID, ALYSSA TEIXEIRA; ANTUNES GARCIA, LUISA HELENA; DE LIMA NASCIMENTO, TATIANA RITA; CANCADO CASTELLANO, LUCIO ROBERTO; SOARES BOMBONATTI, JULIANA FRAGA; HONORIO, HEITOR MARQUES; LIA MONDELLI, RAFAEL FRANCISCO; SAURO, SALVATORE; DE AMOEDO CAMPOS VELO, MARILIA MATTAR. Effects of hybrid inorganic-organic nanofibers on the properties of enamel resin infiltrants - An in vitro study. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 126, p. 10-pg., 2022-02-01. (19/06045-2, 19/10840-2)