A novel peptide-based electrochemical biosensor for breast cancer characterization over a poly 3-(3-aminophenyl) propionic acid matrix

Author(s)

Alves, Rafael da Fonseca ; Martins, Isabella Castro ; Franco, Diego Leoni ; Silva, Adriane das Gracas ; Santos, Paula de Souza ; Goulart, Luiz Ricardo ; de Paiva Maia, Yara Cristina ; Faria Silva, Alinne Tatiane ; Araujo, Thaise Goncalves ; Taboada Sotomayor, Maria Del Pilar

• Document type: Journal article
• Source: BIOSENSORS & BIOELECTRONICS; v. 205, p. 10-pg., 2022-06-01.

Abstract

In this work, a new electrochemical biosensor was developed using peptides selected by Phage Display as bio-recognition phase to Breast Cancer (BC) characterization. Phage clones were selected against MCF-7 (ER-positive BC) proteins, in order to characterize patients with aggressive luminal BC. Biotin-C3 and biotin-H2 peptides were chemically synthesized and validated by flow cytometry, immunofluorescence assays, and ELISA assays, being more reactive to the MCF-7 lineage. Furthermore, a new matrix for the coupling of biomolecules on the surface of graphite electrodes was generated, through electrochemical modification with a new material derived from 3-(3-aminophenyl)propionic acid (3-3-APPA). Electrochemical and morphological characterizations were carried out, and the mechanism of electropolymerization of poly(3-3-APPA) was proposed, in which the carboxylate groups are kept in the structure of the formed polymer. Then, a biosensor was developed by immobilizing the biotin-C3 and biotin-H2 peptides in the SPE/poly(3-3-APPA)/avidin system for the detection of BC tumor markers in serological samples. Finally, peptides were validated using samples from patients with BC and Benign Breast Disease. Biotin-C3 peptide characterized luminal BC according to p53 status and to HER2 expression, being the biosensor a better strategy when compared to ELISA test. This new biosensor will open a new perspective for a rapid and electrochemical platform for the characterization of BC and its molecular subtypes. (AU)

• FAPESP's process: 19/10452-2 - Development of electrochemical biosensor and lateral flow device, low-cost for detection of neoplastic biomarkers
• Grantee: Rafael da Fonseca Alves
• Support Opportunities: Scholarships in Brazil - Doctorate