Effect of 12-wk Training in Ovariectomised Rats on PGC-1 alpha, NRF-1 and Energy Substrates

Author(s)

Melges Pejon, Taciane Maria ; Faria, Vinicius Silva ; Gobatto, Claudio Alexandre ; Manchado-Gobatto, Fulvia Barros ; Menezes Scariot, Pedro Paulo ; Cornachione, Anabelle Silva ; Beck, Wladimir Rafael

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF SPORTS MEDICINE; v. N/A, p. 10-pg., 2022-02-18.

Abstract

Metabolic diseases are associated with hypoestrogenism owing to their lower energy expenditure and consequent imbalance. Physical training promotes energy expenditure through PGC-1 alpha and NRF-1, which are muscle proteins of the oxidative metabolism. However, the influence of physical training on protein expression in individuals with hypoestrogenism remains uncertain. Thus, the aim of this study is to determine the effect of 12 weeks of moderate-intensity swimming training on the muscle expression of PGC-1 alpha, NRF-1, glycogen and triglyceride in ovariectomised rats. OVX and OVX+TR rats were subjected to ovariectomy. The trained animals swam for 30 minutes, 5 days/week, at 80% of the critical load intensity. Soleus was collected to quantify PGC-1 alpha and NRF-1 expressions, while gastrocnemius and gluteus maximus were collected to measure glycogen and triglyceride. Blood glucose was also evaluated. Whereas ovariectomy decreased PGC-1 alpha expression (p<0.05) without altering NRF-1 (p=0.48), physical training increased PGC-1 alpha (p<0.01) and NRF-1 (p<0.05). Ovariectomy reduced glycogen (p<0.05) and triglyceride (p<0.05), whereas physical training increased glycogen (p<0.05) but did not change triglyceride (p=0.06). Ovariectomy increased blood glucose (p<0.01), while physical training reduced it (p<0.01). In summary, 12 weeks of individualized and moderate-intensity training were capable of preventing muscle metabolic consequences caused by ovariectomy. (AU)

• FAPESP's process: 19/08148-3 - Melatonin and physical performance: from ergogenic to accelerated skeletal muscle repair after damage induced by physical exercise
• Grantee: Wladimir Rafael Beck
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/21621-3 - Effect of task specific training on muscle adaptation in gerbils post-ischemic stroke
• Grantee: Thiago Luiz de Russo
• Support Opportunities: Regular Research Grants