| Full text | |
| Author(s): |
Sousa Teixeira, M., V
;
Fernandes, L. M.
;
de Paula, V. Stefanelli
;
Ferreira, A. G.
;
Jacometti Cardoso Furtado, N. A.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | Letters in Applied Microbiology; v. 74, n. 4, p. 8-pg., 2022-01-17. |
| Abstract | |
The incidence of Candida glabrata infections has rapidly grown and this species is among those responsible for causing invasive candidiasis with a high mortality rate. The diterpene ent-hardwickiic acid is a major constituent in Copaifera pubiflora oleoresin and the ethnopharmacological uses of this oleoresin by people from Brazilian Amazonian region point to a potential use of this major constituent as an antimicrobial. Therefore, the goal of this study was to evaluate the antifungal activity of ent-hardwickiic acid against Candida species and to produce derivatives of this diterpene by using microbial models for simulating the mammalian metabolism. The microbial transformations of ent-hardwickiic acid were carried out by Aspergillus brasiliensis and Cunninghamella elegans and hydroxylated metabolites were isolated and their chemical structures were determined. The antifungal activity of ent-hardwickiic acid and its metabolites was assessed by using the microdilution broth method in 96-well microplates and compared with that of fluconazole. All the diterpenes showed fungistatic effects (ranging from 19 center dot 7 to 75 center dot 2 mu mol l(-1)) against C. glabrata at lower concentrations than fluconazole (163 center dot 2 mu mol l(-1)) and were more potent fungicides (ranging from 39 center dot 5 to 150 center dot 4 mu mol l(-1)) than fluconazole, which showed fungicidal effect at the concentration of 326 center dot 5 mu mol l(-1). (AU) | |
| FAPESP's process: | 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species |
| Grantee: | Jairo Kenupp Bastos |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/25201-7 - In vitro studies of bioaccessibility, intestinal transportation and biotransformation of ent-hardwickiic and ent-polyalthic acids from Copaifera pubiflora and Copaifera duckei oil resins |
| Grantee: | Niege Araçari Jacometti Cardoso Furtado |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/20397-3 - Investigation of the potential of filamentous fungi to biotransform the ent-polyalthic acid |
| Grantee: | Lívia Maria Fernandes |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 17/20071-0 - Investigation of the potential of filamentous fungi to biotransform the ent-hardwickiic acid |
| Grantee: | Vinicius Stefanelli de Paula |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |