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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fungal metalloprotease generate whey-derived peptides that may be involved in apoptosis in B16F10 melanoma cells

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Author(s):
Youssef Ali Abou HAMIN NETO [1] ; Nathália Gonsales da Rosa GARZON [2] ; Luciana Barbosa COITINHO [3] ; Lays Martin SOBRAL [4] ; Andréia Machado LEOPOLDINO [5] ; Thaís Regiani CATALDI [6] ; Carlos Alberto LABATE [7] ; Hamilton CABRAL [8]
Total Authors: 8
Affiliation:
[1] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Ciências Farmacêuticas - Brasil
[2] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Ciências Farmacêuticas - Brasil
[3] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Ciências Farmacêuticas - Brasil
[4] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Toxicológicas e Bromatológicas. Departamento de Análises Clínicas - Brasil
[5] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Toxicológicas e Bromatológicas. Departamento de Análises Clínicas - Brasil
[6] Universidade de São Paulo. Escola Superior de Agronomia Luiz de Queiroz. Departamento de Genética - Brasil
[7] Universidade de São Paulo. Escola Superior de Agronomia Luiz de Queiroz. Departamento de Genética - Brasil
[8] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Ciências Farmacêuticas - Brasil
Total Affiliations: 8
Document type: Journal article
Source: FOOD SCIENCE AND TECHNOLOGY; v. 42, 2022-07-26.
Abstract

Abstract Proteases are enzymes that act in the hydrolysis of proteins and have several industrial applications. Moreover, proteases have gained prominence as enzymes for the generation of bioactive peptides from the hydrolysis of different protein sources. Milk is the most studied protein source to obtain peptides due to its nutritional and physiological effects and has been studied as complementary therapeutic approaches for the cancer treatment, interacting specifically with cancer cells, consequently fewer side effects. The ability of Eupenicillium javanicum metalloprotease to generate whey-derived peptides with antioxidant activity has already been demonstrated. For this reason, we thus hypothesized that whey-derived peptides from Eupenicillium javanicum metalloprotease hydrolysis could also have a potential against melanoma cell lines. In this study, B16F10 melanoma cells were treated for 72 h with whey-derived peptides and the effects on cell viability were determined. Moreover, the protein profiles of the treated and nontreated cells were compared in proteomic assay and mass spectrometry analyzes. Whey-derived peptides impaired about 62% cell viability, and proteomic approach associated this behavior to modulate proteins involved in proliferation, energy, apoptosis, metastatic and malignancy rates. This study describes the relevance of microbial enzymes in generation of whey-derived peptides with biological activity against melanoma cells. (AU)

FAPESP's process: 20/14426-3 - Cloning, expression and mapping of non-classical peptidase subsites: biotechnological application in obtaining bioactive peptides
Grantee:Hamilton Cabral
Support Opportunities: Regular Research Grants
FAPESP's process: 12/24703-8 - Proteomics analyses of meso and thermophilic filamentous fungi exposed to physical and chemical factors
Grantee:Hamilton Cabral
Support Opportunities: Regular Research Grants
FAPESP's process: 12/18278-2 - Bioprocess, purification and characterization of peptidases produced by fungi Eupenicillium javanicum and Myceliophthora sp. and evaluating the production of biologically active peptides
Grantee:Youssef Ali Abou Hamin Neto
Support Opportunities: Scholarships in Brazil - Doctorate