Temporomandibular disorder in construction workers associated with ANKK1 and DRD2 genes

Author(s)

Samantha Schaffer Pugsley Baratto ^[1] ; Michelle Nascimento Meger ^[2] ; Vânia Camargo ^[3] ; Gisele Maria Correr Nolasco ^[4] ; Natanael Henrique Ribeiro Mattos ^[5] ; Liliane Roskamp ^[6] ; José Stechman-Neto ^[7] ; Isabela Ribeiro Madalena ; Erika Calvano Küchler ; Flares Baratto-Filho

Affiliation

^[1] UniDomBosco University Center. School of Dentistry - Brasil

^[2] University of Tuiuti of Paraná. School of Dentistry - Brasil

^[3] Positivo University. School of Health Sciences - Brasil

^[4] University of Tuiuti of Paraná. School of Dentistry - Brasil

^[5] University of Tuiuti of Paraná. School of Dentistry - Brasil

^[6] University of Tuiuti of Paraná. School of Dentistry - Brasil

^[7] University of Tuiuti of Paraná. School of Dentistry - Brasil

• Document type: Journal article
• Source: Brazilian Dental Journal; v. 33, n. 4, p. 12-20, 2022-08-26.

Abstract

Abstract The study aimed to explore the influence of genetic polymorphisms in ANKK1 and DRD2 on the signs and symptoms of temporomandibular disorder (TMD) in construction workers. This cross-sectional study included only male subjects. All construction workers were healthy and over 18 years age. Illiterate workers and functionally illiterate workers were excluded. The diagnosis of TMD was established according to the Research Diagnostic Criteria for TMD (RDC/TMD). Genomic DNA was used to evaluate the genetic polymorphisms ANKK1 (rs1800497) and DRD2 (rs6275; rs6276) using Real-Time PCR. Chi-square or Fisher exact tests were used to evaluate genotypes and allele distribution among the studied phenotypes. The established alpha of this study was 5%. The sample included a total of 115 patients. The age of the patients ranged from 19 to 70 years (mean age 38.2; standard deviation 11.7). Chronic pain (87.7%), disc displacement (38.2%), and joint inflammation (26.9%) were the most frequently observed signs and symptoms. The genetic polymorphism rs6276 in DRD2 was associated with chronic pain (p=0.033). In conclusion, our study suggests that genetic polymorphisms in DRD2 and ANKK1 may influence TMD signs and symptoms in a group of male construction workers. (AU)

• FAPESP's process: 15/06866-5 - Evaluation of the role of estrogen in dentofacial development
• Grantee: Erika Calvano Kuchler
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/08149-1 - Evaluation of the role of estrogen in dentofacial tissues
• Grantee: Erika Calvano Kuchler
• Support Opportunities: Scholarships in Brazil - Young Researchers