Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface

Author(s)

Yumi C. Del Rey ^[1] ; Regina G. Palma-Dibb ^[2] ; Rodrigo França ^[3] ; Francisco W.G. Paula-Silva ^[4] ; Débora F.C. Guedes ^[5] ; Cristina Fiuza ^[6] ; Ana C.B.C.J. Fernandes ^[7] ; Juliana J. Faraoni ^[8] ; Lourenço M.R. Roselino ^[9]

Affiliation

^[1] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departmento de Odontologia Restauradora - Brasil

^[2] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departmento de Odontologia Restauradora - Brasil

^[3] University of Manitoba. Faculty of Dentistry. Department of Restorative Dentistry - Canadá

^[4] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departmento de Clínica Infantil - Brasil

^[5] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departmento de Odontologia Restauradora - Brasil

^[6] University of Manitoba. Faculty of Dentistry. Department of Restorative Dentistry - Canadá

^[7] University of Manitoba. Faculty of Dentistry. Department of Oral Biology - Canadá

^[8] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departmento de Odontologia Restauradora - Brasil

^[9] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departmento de Odontologia Restauradora - Brasil

• Document type: Journal article
• Source: Brazilian Dental Journal; v. 33, n. 4, p. 62-70, 2022-08-26.

Abstract

Abstract Proanthocyanidin (PA) is a promising dentin biomodifier due to its ability to stabilize collagen fibrils against degradation by matrix metalloproteinases (MMPs); however, the most effective protocol to incorporate PA into bonding procedures is still unclear. This study evaluated the effect of dentin biomodification with a PA acid etchant on MMP activity, adhesive interface morphology and resin-dentin microtensile bond strength. Sound extracted human molars were flattened to expose dentin and acid-etched for 15 s according to the groups: EXP - experimental phosphoric acid; EXP+PA - experimental phosphoric acid 10% PA; TE - total-etching system; SE - self-etching system. Samples were restored with composite resin and stored in distilled water (37ºC). MMP activity and interface morphology were analyzed after 24 h by in situ zymography (n=6) and scanning electron microscopy (n=3), respectively. The resin-dentin microtensile bond strength (μTBS) was evaluated after 24 h and 6 months storage (n=6). Significantly higher MMP activity was detected in etched dentin compared with untreated dentin (p<0.05), but no difference among acid groups was found. Resin tags and microtags, indicative of proper adhesive system penetration in dentinal tubules and microtubules, were observed along the hybrid layer in all groups. There was no difference in μTBS between 24 h and 6 months for EXP+PA; moreover, it showed higher long-term μTBS compared with TE and EXP (p<0.05). The results suggest that 15 s of biomodification was not sufficient to significantly reduce MMP activity; nonetheless, EXP+PA was still able to improve resin-dentin bond stability compared with total- and self-etching commercial systems. (AU)

• FAPESP's process: 18/05645-3 - The use of an acid etching containing Proanthocyanidin as a cross-linker agent
• Grantee: Yumi Chokyu Del Rey
• Support Opportunities: Scholarships in Brazil - Scientific Initiation