Effect of Xylitol Varnishes on the Inhibition of Demineralization in Vitro

Author(s)

Paula Andery Naves ^[1] ; Alexandre Lima de Moura ^[2] ; Marcela Charantola Rodrigues ^[3] ; Michele Baffi Diniz ^[4] ; Victor Ellias Arana-Chavez ^[5] ; Maísa Camillo Jordão ^[6] ; Cristiane de Almeida Baldini Cardoso ^[7]

Affiliation

^[1] Cruzeiro do Sul University. Post-graduate Program in Dentistry - Brasil

^[2] Cruzeiro do Sul University. Post-graduate Program in Dentistry - Brasil

^[3] Municipal University of São Caetano do Sul. Graduate Program in Health Education - Brasil

^[4] Cruzeiro do Sul University. Post-graduate Program in Dentistry - Brasil

^[5] University of São Paulo. Department of Biomaterials and Oral Biology. Graduate Program in Dentistry - Brasil

^[6] Cruzeiro do Sul University. Post-graduate Program in Dentistry - Brasil

^[7] Cruzeiro do Sul University. Post-graduate Program in Dentistry - Brasil

• Document type: Journal article
• Source: Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 22, 2022-12-05.

Abstract

Abstract Objective: To evaluate the efficacy of xylitol varnishes in the inhibition of enamel demineralization in vitro. Material and Methods: Bovine enamel blocks (n=120) were randomly allocated to four groups (n = 30), and the surface hardness (SH) was measured at baseline. The blocks were treated with the following varnishes: 20% xylitol, 20% xylitol plus F (5% NaF), Duraphat™ (5% NaF, positive control), and placebo (no-F/xylitol, negative control). The varnishes were applied and removed after 6 h of immersion in artificial saliva. The blocks were subjected to pH cycles (demineralization and remineralization for 2 and 22h/day, respectively, for 8 days). Surface and cross-sectional hardnesses were measured to calculate the percentage of SH loss (%SHL) and the integrated loss of the subsurface hardness (ΔKHN). Data were statistically analyzed using Kruskal-Wallis and Tukey’s tests (p<0.05). Results: %SHL was significantly decreased by 20% xylitol plus F, Duraphat™, and 20% xylitol varnishes compared to placebo. The use of 20% xylitol plus F varnish led to a significantly lower percentage of SH loss compared to the use of 20% xylitol varnish without F. However, the experimental and commercial varnishes led to significantly lower subsurface demineralization compared to placebo and did not differ from each other. Conclusion: Xylitol varnishes, especially when combined with F, effectively prevent enamel demineralization. (AU)

• FAPESP's process: 13/09533-1 - XYLITOL- AND/OR FLUORIDE-CONTAINING VARNISHES FOR CARIES CONTROL: IN VITRO, IN SITU AND CLINICAL RANZOMIZED STUDIES.
• Grantee: Cristiane de Almeida Baldini Cardoso
• Support Opportunities: Scholarships in Brazil - Post-Doctoral