| Full text | |
| Author(s): |
Simone Cristina Méo Niciura
[1]
;
Alessandro Pelegrine Minho
[2]
;
Jennifer McIntyre
[3]
;
Magda Vieira Benavides
[4]
;
Cintia Hiromi Okino
[5]
;
Sergio Novita Esteves
[6]
;
Ana Carolina de Souza Chagas
[7]
;
Alessandro Francisco Talamini do Amarante
[8]
Total Authors: 8
|
| Affiliation: | [1] Embrapa Pecuária Sudeste - Brasil
[2] Embrapa Pecuária Sudeste - Brasil
[3] University of Glasgow - Reino Unido
[4] Embrapa Pecuária Sul - Brasil
[5] Embrapa Pecuária Sudeste - Brasil
[6] Embrapa Pecuária Sudeste - Brasil
[7] Embrapa Pecuária Sudeste - Brasil
[8] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Instituto de Biociências - Brasil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | Revista Brasileira de Parasitologia Veterinária; v. 32, n. 1 2023-01-13. |
| Abstract | |
Abstract Haemonchus contortus is a constraint to sheep production. Seeking to reduce the use of hosts and produce parasitic stages in large-scale, a 42-day in vitro culture protocol of H. contortus third-stage larvae was optimized using Dulbecco’s modified Eagle’s medium (DMEM). In cell-free culture, larvae were maintained at 39.6°C, in acidic media (pH 6.1) for 3 or 6 days with Δ4-dafachronic acid followed by DMEM pH 7.4 supplemented or not with Fildes’ reagent. In DMEM pH 7.4 at 37°C, supplementation with Caco-2 cells was compared to Fildes. On Day 14, fourth-stage larvae (L4) development rates in acidic media supplemented (86.8-88.4%) or not (74.4-77.8%) with Fildes and in Caco-2 cell co-culture (92.6%) were similar, and superior to DMEM pH 7.4 with Fildes (0.0%). On Day 21, Caco-2 cell co-culture resulted in higher larvae differentiation (25.0%) and lower degeneration (13.9%) compared to acidic media (1.5-8.1% and 48.6-69.9%, respectively). This is the first report of prolonged in vitro culture of H. contortus larvae using commercial media in co-culture with Caco-2 cells. Although no progression to the adult stage, Caco-2 cell co-culture resulted in morphological differentiation of H. contortus L4 and larval viability for up to 28 days. (AU) | |
| FAPESP's process: | 19/02967-2 - Development of methods to study Haemonchus contortus and anthelmintic resistance to monepantel |
| Grantee: | Simone Cristina Méo Niciura |
| Support Opportunities: | Regular Research Grants |