| Full text | |
| Author(s): |
Franceliusa Delys de Oliveira
[1]
;
Paulo Ramos da Silva Santos
[2]
;
Moacir Franco de Oliveira
[3]
;
Antônio Chaves de Assis Neto
[4]
Total Authors: 4
|
| Affiliation: | [1] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia - Brasil
[2] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia - Brasil
[3] Universidade Federal Rural do Semi-Árido. Departamento de Ciência Animal - Brasil
[4] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia - Brasil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Animal Reproduction; v. 19, n. 4 2023-01-16. |
| Abstract | |
Abstract Estrogens and progestogens are hormones produced by maternal organs and it is required for recognition and maintenance of pregnancy. In addition, the embryo may also be a source. For this, the aim was to identify steroidogenic expression on Galea spixii embryos early in the embryonic period. Embryos were collected on Days 10 and 15 of gestation; some were fixed in 4% paraformaldehyde for morphological and immunohistochemical analysis (P450arom), whereas others had RNA extracted to determine presence of CYP19a1 gene. In addition, for immunochemistry, maternal ovaries were collected as positive control tissues. Maternal tissues had positive staining for aromatase, whereas none of the embryos stained for P450 aromatase. Based on qPCR reactions, CYP19a1 gene were expressed in all embryos. Galea spixii embryos expressed steroidogenic genes during the post-implantation period, indicating they have the potential to produce steroid hormones. (AU) | |
| FAPESP's process: | 13/02535-9 - Steroidogenic control of sexual intrauterine differentiation in Galea spixii |
| Grantee: | Amilton Cesar dos Santos |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/05952-3 - 51st SSR Annual Meeting |
| Grantee: | Antônio Chaves de Assis Neto |
| Support Opportunities: | Research Grants - Meeting - Abroad |
| FAPESP's process: | 12/11217-8 - Expression of steroidogenic enzymes: Cytocromo P40 Aromatases, NADPH reductase and P450c17 during phases of reproductive development and spermatic segments in male captive-bred Spix's yellow-toothed and agouti |
| Grantee: | Antônio Chaves de Assis Neto |
| Support Opportunities: | Regular Research Grants |