Cold Atmospheric Pressure Plasma Is Effective against P. gingivalis (HW24D-1) Mature Biofilms and Non-Genotoxic to Oral Cells

Author(s)

Gouvea Lima, Gabriela de Morais ; Lima Carta, Celina Faig ; Borges, Aline Chiodi ; Castaldelli Nishime, Thalita Mayumi ; Villela da Silva, Cezar Augusto ; Caliari, Marcelo Vidigal ; Alves Mayer, Marcia Pinto ; Kostov, Konstantin Georgiev ; Koga-Ito, Cristiane Yumi

• Document type: Journal article
• Source: APPLIED SCIENCES-BASEL; v. 12, n. 14, p. 11-pg., 2022-07-01.

Abstract

Featured Application Helium cold atmospheric plasma jet is safe to oral cells' DNA in applications from 1 to 7 min. The effects of helium cold atmospheric pressure plasma (He-CAPP) jet on Porphyromonas gingivalis (HW24D-1) biofilm, on human gingival fibroblasts (HGF) and human gingival keratinocytes (OBA-9) were assessed. Standardized suspension of P. gingivalis was obtained, and biofilms were grown anaerobically for 48 h. After exposition to He-CAPP, the biofilm viability was evaluated by XTT assay. HGF were grown at 37 degrees C, in an CO2 chamber in DMEM, while OBA-9 cells were cultured in keratinocyte serum-free medium. After 24 h, plates were exposed to He-CAPP for 1 to 7 min. Plasma was generated using a commercial AC power supply with amplitude modulated signal (voltage amplitude of 20 kVp-p, frequency of 31.0 kHz and duty cycle of 22%). The corresponding discharge power was 0.6W at He flow rate of 1 L/min. DNA damage was accessed by static cytometry. Data were analyzed by GraphPad Prism (p < 0.05). Significant reductions in P. gingivalis viability in relation to non-treated groups were detected (p < 0.0001), directly proportional to exposure time. Treated groups were slightly aneuploid after 5- and 7-min treatment in HGF, and for 3 min in OBA-9 cells, with 1.2 DNA index mean. Helium cold atmospheric pressure plasma jet showed inhibitory effect on P. gingivalis mature biofilm and was not genotoxic for epithelial gingival cells and human oral fibroblasts. (AU)

• FAPESP's process: 16/07196-6 - Prospection of innovative therapeutic alternatives for infectious diseases with relevance in Dentistry: low temperature atmospheric pressure plasma and nanoencapsulate of bioactive substance
• Grantee: Cristiane Yumi Koga Ito
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/03470-3 - Aplications of low temperature atmospheric pressure plasma in dentistry
• Grantee: Gabriela de Morais Gouvêa Lima
• Support Opportunities: Scholarships in Brazil - Post-Doctoral