Evaluating the potential of Pediococcus pentosaceus as a biocontrol agent against tenuazonic acid-producing Alternaria alternata on livestock feeds

Author(s)

de Oliveira, Rodrigo Cardoso ; Nobrega Mendonca, Carlos Miguel ; Verissimo, Nathalia Vieira ; Yokomizo de Almeida, Sonia Regina ; Correa, Benedito ; Watanabe, Ii-Sei ; de Souza de Azevedo, Pamela Oliveira ; de Souza Oliveira, Ricardo Pinheiro

• Document type: Journal article
• Source: Journal of Applied Microbiology; v. 133, n. 5, p. 10-pg., 2022-08-10.

Abstract

Aims This study aims to demonstrate the potential of the lactic acid bacteria (LAB) Pediococcus pentosaceus LBM18 against the mycotoxin-producing Alternaria alternata TEF-1A and highlight its application as an effective grain silage inoculant to control mycotoxin contamination. Methods and Results The antifungal properties of Ped. pentosaceus lyophilized (PPL) were assessed by evaluating its effect on A. alternata TEF-1A grown in a corn silage-based medium, which included morphological changes by Scanning Electron Microscopy (SEM) observations, growth rate, conidia production assays, and inhibition of Tenuazonic acid (TeA) production by high-performance liquid chromatography (HPLC-MS/MS) analyses. Furthermore, TeA biosynthesis was monitored for changes at the molecular level by PKS gene expression. The growth and sporulation processes of A. alternata TEF-1A were affected by Ped. pentosaceus LBM18 in a concentration-dependent manner. Moreover, a significant inhibition of TeA production (74.3%) and the transcription level of the PKS gene (42.9%) was observed. Conclusions Ped. pentosaceus is one of the promising LAB to be applied as an inoculant for corn silage preservation, aiming to inhibit mycotoxigenic fungi growth and their mycotoxin production. Significance and impact of the study Ped. pentosaceus could be used as an inoculant to reduce fungal and mycotoxins contamination in grain silage production. (AU)

• FAPESP's process: 19/16551-2 - Biotechnological product from the use of Pediococcus pentosaceus as a biological additive for the natural conservation of silages
• Grantee: Pamela Oliveira de Souza de Azevedo
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• FAPESP's process: 20/13271-6 - Production of bacteriocin-producing probiotic bacteria
• Grantee: Carlos Miguel Nóbrega Mendonça
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/14144-8 - Purification and encapsulation of bacteriocins from probiotic bacteria for applications in animal protein production systems
• Grantee: Nathalia Vieira Porphirio Veríssimo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/10676-5 - Selection of bacteriocinogenic probiotic strains
• Grantee: Rodrigo Cardoso de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/02456-8 - Tenuazonic acid in Brazilian barley grains: occurrence, fungi, and ecophysiological aspects
• Grantee: Benedito Corrêa
• Support Opportunities: Regular Research Grants