Characterization of Lactiplantibacillus plantarum Tw226 strain and its use for the production of a new membrane-bound biosurfactant

Author(s)

Lara, Virginia M. ; Mendonca, Carlos M. N. ; Silva, Francisco V. S. ; Marguet, Emilio R. ; Vallejo, Marisol ; Converti, Attilio ; Varani, Alessandro M. ; Gliemmo, Maria F. ; Campos, Carmen A. ; Oliveira, Ricardo P. S.

• Document type: Journal article
• Source: JOURNAL OF MOLECULAR LIQUIDS; v. 363, p. 10-pg., 2022-07-26.

Abstract

This study aimed to evaluate the viability for biotechnological uses of a new cell-bound biosurfactant produced by a lactic acid bacterium isolated from the intestinal tract of Odontesthes bonariensis. For this purpose, the bacterial strain was characterized, and the conditions for biosurfactant production were optimized. Moreover, the biosurfactant was chemically characterized, and its possible use as an emulsi-fying agent assessed. The bacterium was identified as Lactiplantibacillus plantarum Tw226 and qualified as safe for biotechnological uses according to the genomic and antibiotic resistance studies. The agitation did not influence the biosurfactant productivity, which reached its maximum value, using MRS medium as a nutrient source, before 48 h of incubation at a temperature of 35 degrees C. The glyco-lipo-peptide nature of the biosurfactant was confirmed by the analysis of its chemical composition. It showed stable surface activity in the pH range of 5-7 and a remarkable functionality as an emulsifier, especially in emulsions containing NaCl. (c) 2022 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 20/13271-6 - Production of bacteriocin-producing probiotic bacteria
• Grantee: Carlos Miguel Nóbrega Mendonça
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Projects - Thematic Grants