Integrated analysis of fish intestine biomarkers: Complementary tools for pollution assessment

Author(s)

Marinsek, Gabriela Pustiglione ; Gusso Choueri, Paloma Kachel ; Choueri, Rodrigo Brasil ; de Souza Abessa, Denis Moledo ; Nascimento Goncalves, Alexandre Rodrigo ; Bortolotto, Lorihany Bogo ; Mari, Renata de Britto

• Document type: Journal article
• Source: Marine Pollution Bulletin; v. 178, p. 11-pg., 2022-03-31.

Abstract

The gastrointestinal tract and its enteric nervous system are the first routes of food and xenobiotics uptake. Considering the importance of this organ, this study evaluated intestinal biomarkers of Sphoeroides testudineus integrating the data to generate tools for pollution assessment. The fish were collected in three sites of Sao Paulo Coast and their intestines were analyzed for biochemical, histology, and neuronal density and morphometry biomarkers. To evaluate the differences among the data, a PERMANOVA was applied, followed by a FA/PCA. The PERMANOVA indicated differences (P < 0.001) between the regions (RA, A1, and A2). Four factors were extracted from the FA/PCA (62% cumulative), showing that the animals from A2 presented severe alterations, mainly in intestinal morphometry and neuronal density. A1 alterations refer mainly to the increase of neuronal metabolism. Our results also evidence a gradient of environmental quality related to the protection level (AR > A1 > A2). (AU)

• FAPESP's process: 14/07793-9 - Assessment of the anthropogenic action on morphofunctional aspects of puffer fish Sphoeroides testudineus (Tetradontiformes: Tetradontidae) in estuarine complex of Santos and Peruíbe, São Paulo
• Grantee: Renata de Britto Mari
• Support Opportunities: Regular Research Grants