Oral Administration of Probiotics (Bacillus subtilis and Lactobacillus plantarum) in Nile Tilapia (Oreochromis niloticus) Vaccinated and Challenged with Streptococcus agalactiae

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Guimaraes, Mateus Cardoso ; Cerezo, Isabel M. ; Fernandez-Alarcon, Miguel Frederico ; Natori, Mariene Miyoko ; Sato, Luciana Yuri ; Kato, Camila A. T. ; Morinigo, Miguel Angel ; Tapia-Paniagua, Silvana ; Dias, Danielle de Carla ; Ishikawa, Carlos Massatoshi ; Ranzani-Paiva, Maria Jose T. ; Cassiano, Luara Lucena ; Bach, Erna Elisabeth ; Clissa, Patricia B. ; Orefice, Daniele P. ; Tachibana, Leonardo

• Document type: Journal article
• Source: FISHES; v. 7, n. 4, p. 18-pg., 2022-08-01.

Abstract

Streptococcus agalactiae is an important bacterial pathogen in intensive Nile tilapia production, causing high mortality rates and great economic losses. This work aimed to evaluate the Nile tilapia vaccination against S. agalactiae and fed with ration containing probiotic AQUA PHOTO(R) composed of Bacillus subtilis and Lactobacillus plantarum, on the immune response action and gut microbiota. The experimental design was completely randomized with five treatments (CON = control; ADJ = adjuvant; PRO = probiotic; VAC = vaccine; PRO + VAC = probiotic + vaccine) and five replicates. The vaccine (bacterin + adjuvant) was injected after 21 days (21d) of probiotic feeding and the vaccine was booster 14 days post-vaccination (35d). After 14 days of the booster (49d), the fish were challenged with S. agalactiae and observed for more than 14 days, completing 63 days. The immunized group showed a better survival rate (CON 40%; ADJ 57%; PRO 67%; VAC 87%; PRO + VAC 97%). The treatments VAC and PRO + VAC, after booster produced higher levels of IgM antibodies compared with the control from the same time. The combination of probiotic and vaccination provided better protection against S. agalactiae infection, directly affecting the gut microbiological profile. These results indicated the contribution of probiotic to the adaptive immune response through the modulation of the intestinal microbiota, improving the effect of the vaccination. In conclusion, AQUA PHOTO(R), composed of B. subtilis and L. plantarum, orally administered to Nile tilapia vaccinated against and challenged with S. agalactiae increases protection from infection and modifies the intestinal microbiota profile of the host, promoting the microbiota balance and improving adaptive immune response. (AU)

• FAPESP's process: 19/20441-8 - Metagenomic analysis of probiotic-fed Nile tilapia, Oreochromis niloticus
• Grantee: Mateus Cardoso Guimarães
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 17/23225-9 - Probiotics and vaccines in raised of Nile tilapia, Oreochromis niloticus
• Grantee: Mateus Cardoso Guimarães
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/03738-1 - Immunostimulation of Nile tilapia, Oreochromis niloticus fed with probiotic and vaccinated against Streptococcus agalactiae
• Grantee: Leonardo Tachibana
• Support Opportunities: Regular Research Grants