Intermittency properties in a temporal lobe epilepsy model

Author(s)

Borges, F. S. ; Gabrickc, E. C. ; Protachevicz, P. R. ; Higa, G. S. V. ; Lameu, E. L. ; Rodriguez, P. X. R. ; Ferraz, M. S. A. ; Szezech Jr, J. D. ; Batista, A. M. ; Kihara, A. H.

• Document type: Journal article
• Source: Epilepsy & Behavior; v. 139, p. 9-pg., 2023-01-16.

Abstract

Neuronal synchronization is important for communication between brain regions and plays a key role in learning. However, changes in connectivity can lead to hyper-synchronized states related to epileptic sei-zures that occur intermittently with asynchronous states. The activity-regulated cytoskeleton-associated protein (ARC) is related to synaptic alterations which can lead to epilepsy. Induction of status epilepticus in rodent models causes the appearance of intense ARC immunoreactive neurons (IAINs), which present a higher number of connections and conductance intensity than non-IAINs. This alteration might con-tribute to abnormal epileptic seizure activity. In this work, we investigated how IAINs connectivity influ-ences the firing pattern and synchronization in neural networks. Firstly, we showed the appearance of synchronized burst patterns due to the emergence of IAINs. Second, we described how the increase of IAINs connectivity favors the appearance of intermittent up and down activities associated with syn-chronous bursts and asynchronous spikes, respectively. Once the intermittent activity was properly char-acterized, we applied the optogenetics control of the high synchronous activities in the intermittent regime. To do this, we considered that 1% of neurons were transfected and became photosensitive. We observed that optogenetics methods to control synchronized burst patterns are effective when IAINs are chosen as photosensitive, but not effective in non-IAINs. Therefore, our analyses suggest that IAINs play a pivotal role in both the generation and suppression of highly synchronized activities.(c) 2022 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 22/09277-4 - Hippocampal pathological plasticity in neonatal anoxia
• Grantee: Guilherme Shigueto Vilar Higa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/16148-5 - Synchronous behaviour and synaptic plasticity in complex networks
• Grantee: Kelly Cristiane Iarosz
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 22/13761-9 - Complex Systems Dynamics
• Grantee: Iberê Luiz Caldas
• Support Opportunities: Research Grants - Visiting Researcher Grant - Brazil
• FAPESP's process: 19/17892-8 - Degeneration and development of the nervous system: the role of epigenetic processes
• Grantee: Alexandre Hiroaki Kihara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/18977-1 - Analysis of Electrical Synapses Contribution in Neuronal Synchronization
• Grantee: Fernando da Silva Borges
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/15024-9 - Information flow in neuronal networks of networks: oscillations, criticality and electrical synapses
• Grantee: Mariana Sacrini Ayres Ferraz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/04624-2 - Synaptic plasticity in neuronal networks
• Grantee: Paulo Ricardo Protachevicz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/19296-1 - Nonlinear dynamics
• Grantee: Iberê Luiz Caldas
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/07311-7 - Dynamic behaviour of neural networks
• Grantee: Kelly Cristiane Iarosz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral