| Full text | |
| Author(s): |
Cristina-Silva, Caroline
;
Amaral-Silva, Lara
;
Santos, Kassia Moreira
;
Correa, Gabriela Monteiro
;
da Silva, Welex Candido
;
Fernandes, Marcia H. M. R.
;
da Silva, Glauber S. F.
;
Gargaglioni, Luciane H.
;
Almeida, Maria C.
;
Bicego, Kenia C.
Total Authors: 10
|
| Document type: | Journal article |
| Source: | FRONTIERS IN PHYSIOLOGY; v. 13, p. 13-pg., 2022-07-15. |
| Abstract | |
Transient receptor potential vanilloid 4 (TRPV4) channels are sensitive to warm ambient temperatures (T(a)s), triggering heat loss responses in adult rats in a T(a)s range of similar to 26-30 degrees C. In birds, however, the thermoregulatory role of TRPV4 has never been shown. Here, we hypothesized that stimulation of TRPV4 induces thermolytic responses for body temperature (T-b) maintenance in birds, and that this function is already present in early life, when the T-a range for TRPV4 activation does not represent a warm condition for these animals. We first demonstrated the presence of TRPV4 in the dorsal and ventral skin of chickens (Gallus gallus domesticus) by immunohistochemistry. Then, we evaluated the effects of the TRPV4 agonist, RN1747, and the TRPV4 antagonists, HC067047 and GSK2193874, on T-b and thermoeffectors at different T(a)s in 5-day-old chicks and 60-day-old adult chickens. For the chicks, RN1747 transiently reduced T-b both in thermoneutrality (31 degrees C) and in a cold T-a for this phase (26 degrees C), which relied on huddling behavior inhibition. The TRPV4 antagonists alone did not affect T-b or thermoeffectors but blocked the T-b decrease and huddling inhibition promoted by RN1747. For the adults, TRPV4 antagonism increased T-b when animals were exposed to 28 degrees C (suprathermoneutral condition for adults), but not to 19 degrees C. In contrast, RN1747 decreased T-b by reducing metabolic rate and activating thermal tachypnea at 19 degrees C, a T-a below the activation range of TRPV4. Our results indicate that peripheral TRPV4 receptors are functional in early life, but may be inhibited at that time when the range of activation (similar to 26-30 degrees C) represents cold T-a for chicks, and become physiologically relevant for T-b maintenance when the activation T-a range for TRPV4 becomes suprathermoneutral for adult chickens. (AU) | |
| FAPESP's process: | 17/17278-2 - ROLE OF THE RAPHE IN THE CONTROL OF THERMOEFECTORS IN PRECOCIOUS BIRDS |
| Grantee: | Caroline Cristina Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/12627-9 - Mechanisms involved in the biphasic thermoregulatory response to systemic inflammation in chicks: an integrated approach from the system to the mitochondria |
| Grantee: | Kênia Cardoso Bícego |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/00864-6 - Physiological and biochemical mechanisms of thermal response to systemic inflammation in chicks |
| Grantee: | Lara do Amaral Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/01796-7 - Mechanisms involved in the biphasic thermoregulatory response to systemic inflammation in chicks: an integrated approach of the system to mitochondria |
| Grantee: | Welex Cândido da Silva |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 15/04849-6 - Role of TRPV4 channels in thermoregulation in endotherms |
| Grantee: | Kênia Cardoso Bícego |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/06211-0 - Mechanisms involved in the biphasic thermoregulatory response to systemic inflammation in chicks: an integrated approach of the system to mitochondria |
| Grantee: | Welex Cândido da Silva |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 15/02991-0 - Involvement of TRPM8 channels in thermoregulation of Wistar rats |
| Grantee: | Maria Camila Almeida |
| Support Opportunities: | Regular Research Grants |