Bioavailability evaluation of calcium, magnesium and zinc in Brazilian cheese through a combined model of in vitro digestion and Caco-2 cells

Author(s)

da Paixao Teixeira, Jose Luan ; Lima Pallone, Juliana Azevedo ; Delgado Andrade, Cristina ; Mesias, Marta ; Seiquer, Isabel

• Document type: Journal article
• Source: Journal of Food Composition and Analysis; v. 107, p. 9-pg., 2022-04-01.

Abstract

Cheese has important nutritional properties as a result of their protein, lipid and essential minerals content. This study analyzed the bioavailability of Ca, Mg and Zn in Brazilian cheeses, using the in vitro INFOGEST digestion method coupled with assays in Caco-2 cells. Furosine content (marker of the early Maillard reaction) was also evaluated. Two self-produced fresh cheeses, cow and goat Minas frescal; and two commercial matured goat cheeses, Blue and Pyramid, were analyzed. Mineral bioaccessibility after in vitro digestion was in the range of 44.4-74.7 %, 54.8-66.1 % and 18.2-38.7 % for Ca, Mg and Zn, respectively. Bioaccessibilty was significantly affected by the mineral and fat content level. The digested fresh cheeses (Minas frescal) showed the highest values of soluble Ca and Mg; and the Pyramid goat cheese presented the greatest Ca and Zn transport efficacy across Caco-2 cell monolayers. Fumsine could be a useful indicator to evaluate cheese ripeness and its indirect effect on the mineral availability. (AU)

• FAPESP's process: 18/08864-8 - Minerals bioacessibility in goat dairy produts and evaluation of applying NIR and chemometrics to detect adulterations in goat's milk and dairy
• Grantee: José Luan da Paixão Teixeira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/09759-3 - Higher value-added foods: in vitro digestion simulation for minerals bioacessibility estimation and detection of adulterations by infrared spectroscopy and chemiometry
• Grantee: Juliana Azevedo Lima Pallone
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/13600-2 - Study of minerals bioavailability in goat cheese using different methods
• Grantee: José Luan da Paixão Teixeira
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate