| Full text | |
| Author(s): |
Ishida, Juliane K.
;
Bini, Andressa P.
;
Creste, Silvana
;
Van Sluys, Marie-Anne
Total Authors: 4
|
| Document type: | Journal article |
| Source: | BMC Microbiology; v. 22, n. 1, p. 16-pg., 2022-08-08. |
| Abstract | |
Background Plant microbiome and its manipulation inaugurate a new era for plant biotechnology with the potential to benefit sustainable crop production. Here, we used the large-scale 16S rDNA sequencing analysis to unravel the dynamic, structure, and composition of exophytic and endophytic microbial communities in two hybrid commercial cultivars of sugarcane (R570 and SP80-3280), two cultivated genotypes (Saccharum officinarum and Saccharum barberi) and one wild species (Saccharum spontaneum). Results Our analysis identified 1372 amplicon sequence variants (ASVs). The microbial communities' profiles are grouped by two, root and bulk soils and stem and leave when these four components are compared. However, PCoA-based data supports that endophytes and epiphytes communities form distinct groups, revealing an active host-derived mechanism to select the resident microbiota. A strong genotype-influence on the assembly of microbial communities in Saccharum ssp. is documented. A total of 220 ASVs persisted across plant cultivars and species. The ubiquitous bacteria are two potential beneficial bacteria, Acinetobacter ssp., and Serratia symbiotica. Conclusions The results presented support the existence of common and cultivar-specific ASVs in two commercial hybrids, two cultivated canes and one species of Saccharum across tissues (leaves, stems, and roots). Also, evidence is provided that under the experimental conditions described here, each genotype bears its microbial community with little impact from the soil conditions, except in the root system. It remains to be demonstrated which aspect, genotype, environment or both, has the most significant impact on the microbial selection in sugarcane fields. (AU) | |
| FAPESP's process: | 16/17545-8 - Genes, genomes & transposable elements contribution to plant-microbe interaction: a sugarcane study case |
| Grantee: | Marie-Anne Van Sluys |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 18/07615-4 - Studying the first cell communication signals between sugarcane and pathogens: focus on Reactive Oxygen Species. |
| Grantee: | Andressa Peres Bini |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |