Molecular phenotypes of bovine blastocyst derived from in vitro-matured oocyte supplemented with PAPP-A

Author(s)

Dellaqua, Thaisy Tino ; Franchi, Fernanda Fagali ; dos Santos, Priscila Helena ; Giroto, Alan Brunholi ; Nunes, Sarah Gomes ; de Lima, Victor Augusto Vieira ; Guilherme, Vitoria Bertogna ; Fontes, Patricia Kubo ; Sudano, Mateus Jose ; Castilho, Anthony Cesar de Souza

• Document type: Journal article
• Source: VETERINARY RESEARCH COMMUNICATIONS; v. N/A, p. 10-pg., 2023-01-18.

Abstract

Insulin-like growth factor-1 (IGF-1) regulates cellular lipid content, whereas pregnancy-associated plasma protein-A (PAPP-A) increases IGF-1 bioavailability. Using in vitro-matured cumulus-oocyte complexes, we aimed to evaluate the impact of PAPP-A on the blastocyst lipid content, embryo cryotolerance and embryonic transcriptional profile. We determined that PAPP-A did not affect the lipid content of oocytes, blastocysts, or blastocyst yield (P > 0.05). However, PAPP-A modulated the embryo transcriptional profiles by downregulating PPARGC1A and AKR1B1, which are related to lipid metabolism; CASP9, a pro-apoptotic gene; and IFN-tau, a marker of embryo quality (P < 0.05). Furthermore, the use of PAPP-A improved blastocyst re-expansion in the first 3 h of culture after vitrification (P < 0.05). Although PAPP-A did not affect the blastocyst lipid content or embryo production, we suggest that embryonic transcriptional modulation could contribute to maintain the balance in embryo lipid metabolism. Furthermore, PAPP-A's approach seems to control key intracellular pathways that improve post-cryopreservation development of blastocysts. (AU)

• FAPESP's process: 17/19679-4 - Effect of Pregnancy-Associated Plasma Protein- A (PAPP-A) about profile and lipid metabolism of bovine oocytes matured in vitro
• Grantee: Thaisy Tino Dellaqua
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/06454-7 - Impact of pregnancy-associated plasma protein- a (PAPP-A) addition during in vitro oocyte maturation on the profile and lipid metabolism of bovine embryos
• Grantee: Victor Augusto Vieira de Lima
• Support Opportunities: Scholarships in Brazil - Scientific Initiation