| Full text | |
| Author(s): |
Correa, Victor Araujo
;
Portilho, Amanda Izeli
;
De Gaspari, Elizabeth
Total Authors: 3
|
| Document type: | Journal article |
| Source: | DISEASES; v. 10, n. 3, p. 17-pg., 2022-09-01. |
| Abstract | |
The meningococcal disease is a global health threat, but is preventable through vaccination. Adjuvants improve meningococcal vaccines and are able to trigger different aspects of the immune response. The present work evaluated the immune response of mice against Neisseria meningitidis outer membrane vesicles (OMV) complexed with the adjuvants aluminium hydroxide (AH), via subcutaneous route; and dimethyldioctadecylammonium bromide (DDA) or Saponin (Sap), via intranasal/subcutaneous routes. ELISA demonstrated that all adjuvants increased IgG titers after the booster dose, remaining elevated for 18 months. Additionally, adjuvants increased the avidity of the antibodies and the bactericidal titer: OMVs alone were bactericidal until 1:4 dilution but, when adjuvanted by Alum, DDA or Sap, it increased to 1/32. DDA and Sap increased all IgG isotypes, while AH improved IgG1 and IgG2a levels. Thus, Sap led to the recognition of more proteins in Immunoblot, followed by DDA and AH. Sap and AH induced higher IL-4 and IL-17 release, respectively. The use of adjuvants improved both cellular and humoral immune response, however, each adjuvant contributed to particular parameters. This demonstrates the importance of studying different adjuvant options and their suitability to stimulate different immune mechanisms, modulating the immune response. (AU) | |
| FAPESP's process: | 12/15568-0 - Study of the immunogenicity of outer membrane antigens of Neisseria meningitidis and interaction with cationic lipids: evaluation of different immunization schedules in different strains of mice |
| Grantee: | Elizabeth Natal de Gaspari |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/02042-9 - Evaluation of response profiles of Th1 and Th2 in mice immunized with anionic and cationic lipids complexed the outer membrane vesicles of Neisseria meningitis b |
| Grantee: | Victor Araujo Correa |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 18/04202-0 - Study of the immunogenicity of outer membrane antigens of Neisseria meningitidis and interaction with cationic lipids: evaluation of new adjuvant for Neisseria meningitidis in different strains of elderly mice |
| Grantee: | Elizabeth Natal de Gaspari |
| Support Opportunities: | Regular Research Grants |