| Full text | |
| Author(s): |
Canella, Paula Renata Bueno Campos
;
Vinces, Salvador Sanchez
;
Silva, alex Aparecido Rosini
;
Sanches, Pedro Henrique Godoy
;
Barini, Ricardo
;
Porcari, Andreia de Melo
;
Razolli, Daniela Soares
;
Carvalho, Patricia de Oliveira
Total Authors: 8
|
| Document type: | Journal article |
| Source: | American Journal of Reproductive Immunology; v. 89, n. 3, p. 10-pg., 2023-01-11. |
| Abstract | |
Background: Recurrent Pregnancy Loss (RPL) and Recurrent Implantation Failure (RIF) are highly heterogeneous condition and many of the mechanisms involved still require elucidation. The aim was to analyze the lipidomic profile in plasma of women with RPL and RIF before and after receiving the Lipid Emulsion Therapy (LET) containing 10% fish oil (SMOFlipid((R)) 20%). Methods: This study included twenty-six women with RPL or RIF from immunological or inflammatory causes, with elevated natural killer cell levels and divided into a Pregnancy Loss or a Live Birth group according to the outcome. The women received intravenous LET and sample collecting was done before the first, third and fifth dose of LET in the pregnant women. Ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF MS) and multivariate statistical methods were performed to evaluate the profile of phospholipids present in the women's plasma. Results: An increase of phosphatidylcholines (PC) 40:8 and 36:5 levels with predominance of n6 polyunsaturated fatty acids (PUFA) was observed in plasma lipids of the Pregnancy Loss Group compared to Live Birth Group. We also observed an increase in the relative abundance of n3 PUFA-PC species (42:10 and 36:6) and LysoPC 15:0 with the long term use of LET. Conclusion: The greater availability of n3 PUFA in plasma of the pregnant women stemming from LET use can be considered advantageous regarding the alteration of the phospholipid profile and its postulated anti-inflammatory and immunomodulatory role. (AU) | |
| FAPESP's process: | 20/11461-2 - Multivariate platforms for lipidomic study in biological samples |
| Grantee: | Gustavo Henrique Bueno Duarte |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |