QTL and eQTL mapping associated with host response toCandidatusLiberibacter asiaticus in citrandarins

Author(s)

Soratto, Tatiany Aparecida Teixeira ; Curtolo, Maiara ; Marengo, Samanta ; Dezotti, Ana Lucia ; Lima, Romulo Pedro Macedo ; Gazaffi, Rodrigo ; Machado, Marcos Antonio ; Cristofani-Yaly, Mariangela

• Document type: Journal article
• Source: ROPICAL PLANT PATHOLOG; v. 45, n. 6, p. 20-pg., 2020-05-29.

Abstract

Huanglongbing (HLB) is a severe disease of citrus caused by the bacteriumCandidatusLiberibacter. In America, the most common species isCandidatusLiberibacter asiaticus (CLas). In a previous study of the Citrus-HLB pathosystem, our group found differences in CLas multiplication in a population of hybrids obtained by hybridization betweenCitrus sunkiHort. Ex Tan. andPoncirus trifoliata(L.) Raf. cv. Rubidoux. The bacterial concentration was higher inC. sunkithan inP. trifoliata. Thus, this study aims to map phenotypic (QTL) and gene expression (eQTL) data associated with host response to CLas in the linkage groups (LGs) of the previously constructed parental maps ofC. sunkiandP. trifoliatacv. Rubidoux. For the phenotypic analysis, we used a population of 79 F1 hybrids (termed citrandarins) betweenC. sunkiandP. trifoliata. CLas and starch were quantified in the leaves of the plants two years after pathogen inoculation, allowing the classification of hybrids as resistant, tolerant, and susceptible. The expression of 14 candidate genes was measured in 72 hybrids of the population and used as expression data for the eQTL mapping. We located nine QTL and 52 eQTL in theC. sunkimap and 17 QTL and 40 eQTL in theP. trifoliatamap. The overlap of eQTL of the majority of genes with QTL from the phenotypic data indicates that the genes are related to the phenotype and are probably related to pathogen infection. (AU)

• FAPESP's process: 14/50880-0 - INCT 2014: comparative and functional genomics and citrus-assisted breeding
• Grantee: Marcos Antonio Machado
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/22133-0 - Genome editing aimed at QTLs validation associated with tolerance Candidatus Liberibacter asiaticus in citrandarins (Citrus sunki x Poncirus trifoliata)
• Grantee: Maiara Curtolo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/00133-4 - Trials with citrandarins as rootstock for the Pêra and Valencia sweet oranges and the Tahiti acid lime
• Grantee: Mariângela Cristofani-Yaly
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/18605-0 - Obtaining and evaluation of new varieties of scion and rootstock for citrus
• Grantee: Mariângela Cristofani-Yaly
• Support Opportunities: Research Projects - Thematic Grants