| Full text | |
| Author(s): Show less - |
da Rocha, Alisson L.
;
Pinto, Ana P.
;
Bedo, Bruno L. S.
;
Morais, Gustavo P.
;
Oliveira, Luciana C.
;
Carolino, Ruither O. G.
;
Pauli, Jose R.
;
Simabuco, Fernando M.
;
de Moura, Leandro P.
;
Ropelle, Eduardo R.
;
Cintra, Dennys E.
;
Rivas, Donato A.
;
da Silva, Adelino S. R.
Total Authors: 13
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 12, n. 1, p. 13-pg., 2022-11-21. |
| Abstract | |
The transcriptional repressor REV-ERB-alpha, encoded by Nuclear Receptor Subfamily 1 Group D Member 1 (Nr1d1), has been considered to play an essential role in the skeletal muscle oxidative capacity adaptation and muscle mass control. Also, this molecule regulates autophagy via the repression of autophagy-related genes both in skeletal muscle and brain regions. Classically, training programs based on endurance or strength characteristics enhance skeletal muscle mass content and/or oxidative capacity, leading to autophagy activation in several tissues. Thus, it seems that REV-ERB-alpha regulates similar responses induced by exercise. However, how this molecule responds to different exercise models/intensities in different tissues is still unclear. Therefore, the main aim was to characterize the responses of REV-ERB-alpha and autophagy-related genes to different exercise protocols (endurance/interval run/strength) in distinct tissues (gastrocnemius, soleus and hippocampus). Since REV-ERB-alpha presents a circadian rhythm, the analyses were performed in a time-course manner. The endurance and strength groups attenuated REV-ERB-alpha transcriptional response during the time course in gastrocnemius and soleus. Conversely, the interval group enhanced the Nr1d1 expression in the hippocampus. All protocols downregulated the REV-ERB-alpha protein levels in gastrocnemius following the exercise session with concomitant nuclear exclusion. The major autophagy-related genes presented downregulation after the exercise session in all analyzed tissues. Altogether, these results highlight that REV-ERB-alpha is extremely sensitive to physical exercise stimuli, including different models and intensities in skeletal muscle and the hippocampus. (AU) | |
| FAPESP's process: | 21/08693-1 - Effect of global or conditional deletion (skeletal muscle) of the Nr1d1 gene on aging |
| Grantee: | Ana Paula Pinto |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 19/17532-1 - Physical exercise as a non-pharmacological strategy in the prevention and treatment of Alzheimer's Disease |
| Grantee: | Gustavo Paroschi Morais |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/25492-4 - Effects of the combination of intermittent fasting with physical exercise on the hypothalamic autophagy pathway in obese mice |
| Grantee: | Luciana da Costa Oliveira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 19/11820-5 - Nr1d1 function on the aging-associated Sarcopenia |
| Grantee: | Adelino Sanchez Ramos da Silva |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/19869-8 - Molecular mechanisms related to increased hepatic fat content in response to excessive physical exercise |
| Grantee: | Ana Paula Pinto |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/12765-2 - Emerging role of rev-erb-alpha in molecular adaptations to different physical exercise models |
| Grantee: | Alisson Luiz da Rocha |
| Support Opportunities: | Scholarships in Brazil - Doctorate |