The NO-cGMP-K (+) Channel Pathway Participates in Diuretic and Cardioprotective Effects of Blutaparon portulacoides in Spontaneously Hypertensive Rats

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Terco Leite, Patricia Regina ; Lorencone, Bethania Rosa ; Tafarelo Moreno, Karyne Garcia ; Lopes, Katiana Simoes ; Macedo Marques, Aline Aparecida ; Fortini, Clara Soligo ; Calloi Palozi, Rhanany Alan ; Dalmagro, Mariana ; Leite Kassuya, Candida Aparecida ; dos Santos, Ariany Carvalho ; Salvador, Marcos Jose ; Gasparotto Junior, Arquimedes

• Document type: Journal article
• Source: Planta Medica; v. 88, n. 13, p. 11-pg., 2022-03-17.

Abstract

Blutaparon portulacoides is a Brazilian plant species that is widely used in folk medicine. The present study investigated the role of an aqueous extract of B. portulacoides against hypertension in spontaneously hypertensive rats. The aqueous extract of B. portulacoides was obtained from the whole plant. Its chemical profile was analyzed by ultraperformance liquid chromatography-tandem mass spectrometry. The acute toxicity of the aqueous extract of B. portulacoides was evaluated in female Wistar rats. Male 6-month-old spontaneously hypertensive rats then received the aqueous extract of B. portulacoides (30, 100, and 300 mg/kg), hydrochlorothiazide (25 mg/kg), or vehicle once daily for 28 days. On days 1, 14, and 28, the diuretic effects of the aqueous extract of B. portulacoides were evaluated. The role of prostaglandins and the nitric oxide-cyclic guanosine monophosphate-potassium channel pathway in the diuretic activity of the aqueous extract of B. portulacoides was also investigated. At the end of the treatment, hepatic and renal biochemical markers, serum nitrotyrosine, malondialdehyde, nitrite, and aldosterone levels, and angiotensin-converting enzyme activity were measured. The electrocardiographic profile, blood pressure, and renal vascular reactivity were also assessed. The heart, kidneys, and liver were collected to determine relative organ weight, histopathology, and cardiac morphometry. Caffeic acid, ferulic acid, and several flavonoids were identified in the aqueous extract of B. portulacoides . No signs of toxicity were observed. Prolonged treatment with the aqueous extract of B. portulacoides (300 mg/kg) induced significant diuretic activity by activating the nitric oxide-cyclic guanosine monophosphate-potassium channel pathway. These effects reduced blood pressure and oxidative stress and prevented renal vascular dysfunction and left ventricular hypertrophy that was induced by hypertension. Overall, the present data suggest that the aqueous extract of B. portulacoides has important diuretic and cardioprotective effects by activation of the nitric oxide-cyclic guanosine monophosphate-potassium channel pathway. (AU)

• FAPESP's process: 06/06079-4 - Search of antioxidants and antimicrobials in Gomphreneae tribe species
• Grantee: Marcos José Salvador
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/03726-8 - Integrated chemical and biological study in the search for bioactive substances in plants* and development of analytical procedures. *(Amaranthaceae, Gesneriaceae, Myrtaceae e Annonaceae)
• Grantee: Marcos José Salvador
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/09101-1 - Investigation of treatment with 24-epibrassinolide in plant development, profile of volatile constituents and biological activities of essential oils from Cymbopogon citratus, Cymbopogon flexuosus and Mentha pulegium
• Grantee: Marcos José Salvador
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/03598-5 - Preventive effect of Ilex paraguariensis preparations (chimarrão, terere and mate tea) against damages caused by chronic ethanol consumption: comparative analysis of preparation technology, chemical profile and evaluation of therapeutic effect and safety
• Grantee: Mariana Mesquita Fonseca
• Support Opportunities: Scholarships in Brazil - Doctorate